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5,15-DPP (Synonyms: 5,15-Diphenylporphyrin,STAT3 Inhibitor VIII)

Catalog No.GC12138

5,15-DPP (5,15-DPP) is a selective STAT3-SH2 antagonist (IC50s of 0.28 μM and 10 μM for STAT3 and STAT1, respectively).

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5,15-DPP Chemical Structure

Cas No.: 22112-89-6

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25mg
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50mg
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

5,15-diphenylporphyrin (5,15-DPP) is a selective STAT3-SH2 antagonist [1].

Stat3 is constitutively activated in many human cancers. Stat3 functions as a critical mediator of oncogenic signaling through transcriptional activation of genes encoding apoptosis inhibitors, cell-cycle regulators and inducers of angiogenesis [2]. Aberrant STAT3 activity has been associated with transforming mechanisms induced by oncogenic tyrosine kinases [1].

In vitro: 5,15-DPP directly bound to STAT3 and antagonized the function of STAT3-SH2. 5,15-DPP selectively antagonized STAT3-SH2 with an IC50 of 0.28 μM over the other SH2-containing proteins STAT1 and Grb2[1]. The estimated KD values for the 5,15-DPP binding to STAT3 was 880 nM. Treatment with 5,15-DPP suppressed the DNA binding activity of STAT3 in a concentration-dependent manner. 5,15-DPP poorly inhibited STAT1 with an IC50 of 10 μM and showed no effect Grb2 [1].

References:
[1] Uehara Y, Mochizuki M, Matsuno K, et al.  Novel high-throughput screening system for identifying STAT3–SH2 antagonists[J]. Biochemical and biophysical research communications, 2009, 380(3): 627-631.
[2] Jing N, Tweardy D J.  Targeting Stat3 in cancer therapy[J]. Anti-cancer drugs, 2005, 16(6): 601-607.

Chemical Properties

Cas No. 22112-89-6 SDF
Synonyms 5,15-Diphenylporphyrin,STAT3 Inhibitor VIII
Chemical Name 5,15-diphenyl-21H,23H-porphine
Canonical SMILES C1(C=C/2)=NC2=C/C3=CC=C(/C(C4=CC=CC=C4)=C5N=C(/C=C6N/C(C=C\6)=C1/C7=CC=CC=C7)C=C\5)N3
Formula C32H22N4 M.Wt 462.6
Solubility ≤2mg/ml in dimethyl formamide Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 2.1617 mL 10.8085 mL 21.6169 mL
5 mM 0.4323 mL 2.1617 mL 4.3234 mL
10 mM 0.2162 mL 1.0808 mL 2.1617 mL
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