5-Ethynyl-2'-deoxyuridine (Synonyms: 5-EdU, EdU, 2'-deoxy-5-Ethynyluridine) |
Catalog No.GC42504 |
5-ethynyl-2′-deoxyuridine (EdU), as a thymidine analoghas, a terminal alkyne group replacing a methyl group at the 5 position of the pyrimidine ring and can be readily incorporated into DNA during synthesis[1].
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 61135-33-9
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment [1]: | |
Cell lines |
HeLa cells |
Preparation Method |
HeLa cells were incubated with either 10 µM EdU or 10 µM EdC for 4 h. Then, the detection of EdU and DNA using DAPI was performed. |
Reaction Conditions |
10 µM; 4h |
Applications |
The result shown approximately 75% of the cells contained a signal corresponding to the localization of incorporated EdU. |
Animal experiment [2]: | |
Animal models |
bird |
Preparation Method |
In order to label mitotically active supporting cells, a single, subcutaneous injection of EdU (50 mg/kg) in sterile, phosphate buffered saline (PBS, pH 7.4) was administered to each bird 72h after the gentamicin injection. |
Dosage form |
50 mg/kg; s.c. |
Applications |
When EdU is injected at 72h and allowed to incorporate for 4h, some of the supporting cells that first entered S phase around 65h are labeled with EdU at the end of their S phase. They are able to reach the mitotic phase and some even divide during this time. |
References: LigasovÁ A, et al. Dr Jekyll and Mr Hyde: a strange case of 5-ethynyl-2'-deoxyuridine and 5-ethynyl-2'-deoxycytidine. Open Biol. 2016 Jan;6(1):150172. |
5-ethynyl-2′-deoxyuridine (EdU), as a thymidine analoghas, a terminal alkyne group replacing a methyl group at the 5 position of the pyrimidine ring and can be readily incorporated into DNA during synthesis[1]. EdU is a inhibitor of the cell growth of human breast cancer cells (MCF-7 and MDA-MP-231) with the IC50 of 0.4 μM for MCF-7 cells and 4.4 μM for MDA-MB-231 cells[2].
In vitro, 10 μMEdU induced gamma-H2AX foci for 51D1 and KO40 in CHO cells for DNA damage responses[3]. In vitro experiment it shown that A549 cells were treated with 20 μM EdU for 6 h increased the level of expression of γH2AX and ATM-S1981P by 88% and 116%, respectively[4].
In vivo efficacy test it shown that femalJKe mice were administrated 10, 20, 50, 100 or 200 mg/kg EdU intraperitoneally, there is an increase in the number of EdU positive cells in the DG slightlyin a dose-dependent manner[5]. In vivo, few EdU-positive cells were observed in Wistar and GK rats at a dose of 5 mg/kg and 25 mg/kg EdU intraperitoneally. Prominent EdU-positive cells were observed in Wistar and GK rats at a dose of 100 mg/kg and 200 mg/kg, respectively[6]. In vivo, use of 5-Ethynyl-2'-deoxyuridine (EdU) incorporation to in vivo monitor T lymphocyte proliferation by flow cytometry with an adoptive transfer model. The result shown that the percentage of EdU-positive cells increased in a dose-dependent manner, and the saturated dose of EdU was 20mg/kg. Intraperitoneal and intravenous injection had no differences in lymphocyte proliferation detection with EdU in vivo[7].
References:
[1] Kaiser CL, et al. 5-Ethynyl-2'-deoxyuridine labeling detects proliferating cells in the regenerating avian cochlea. Laryngoscope. 2009 Sep;119(9):1770-5.
[2] Meneni S, et al. (2007) 5-Alkynyl-2′-deoxyuridines: chromatography-free synthesis and cytotoxicity evaluation against human breast cancer cells. Bioorg Med Chem 15: 3082–3088.
[3] Haskins JS, et al. Evaluating the Genotoxic and Cytotoxic Effects of Thymidine Analogs, 5-Ethynyl-2'-Deoxyuridine and 5-Bromo-2'-Deoxyurdine to Mammalian Cells. Int J Mol Sci. 2020 Sep 10;21(18):6631.
[4] Zhao H, et al. DNA damage signaling, impairment of cell cycle progression, and apoptosis triggered by 5-ethynyl-2'-deoxyuridine incorporated into DNA. Cytometry A. 2013 Nov;83(11):979-88.
[5] Zeng C, et al. Evaluation of 5-ethynyl-2'-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system. Brain Res. 2010 Mar 10;1319:21-32.
[6] Guo J, Li D, et al. Detecting DNA synthesis of neointimal formation after catheter balloon injury in GK and in Wistar rats: using 5-ethynyl-2'-deoxyuridine. Cardiovasc Diabetol. 2012 Dec 13;11:150.
[7] Sun X, Zhang C, et al. Flow cytometric analysis of T lymphocyte proliferation in vivo by EdU incorporation. Int Immunopharmacol. 2016 Dec;41:56-65.
Cas No. | 61135-33-9 | SDF | |
Synonyms | 5-EdU, EdU, 2'-deoxy-5-Ethynyluridine | ||
Canonical SMILES | O=C(NC(C(C#C)=C1)=O)N1[C@H]2C[C@H](O)[C@@H](CO)O2 | ||
Formula | C11H12N2O5 | M.Wt | 252.2 |
Solubility | DMF: 20 mg/mL,DMSO: 20 mg/mL; Water: 8 mg/mL (31.72 mM; ultrasonic and warming and heat to 37°C) | Storage | 4°C, protect from light |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 3.9651 mL | 19.8255 mL | 39.6511 mL |
5 mM | 0.793 mL | 3.9651 mL | 7.9302 mL |
10 mM | 0.3965 mL | 1.9826 mL | 3.9651 mL |
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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