ABTS diammonium salt (AzBTS-(NH4)2) |
Catalog No.GC33439 |
ABTS diammonium salt (AzBTS-(NH4)2) is a substrate for horseradish peroxidase (HRP) conjugate.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 30931-67-0
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Kinase experiment: |
Enzyme activity is determined photometrically using a temperature controlled multi-mode plate reader or alternatively in a UV/Vis spectrophotometer. Reactions are initiated by addition of the enzyme. Enzyme activity is measured over a period of 10 min at 25°C at the appropriate wavelength for the substrate. One unit (1 U) is defined as the amount of enzyme that converts 1 µmol substrate per minute. Various H2O2 concentrations (0-1250 µM, enzyme concentrations (0.27-54 nM) and substrate concentrations are used to determine the enzyme activity. The activity of rPsaDyP vs ABTS is determined in 100 mM sodium acetate buffer at pH 3.8 and a final H2O2 concentration of 125 µM. Production of the ABTS cation radical is studied at 420 nm (ε420 36,000 L mol-1 cm-1)[2]. |
References: [1]. Matsuda H, et al. Evaluation of ELISA with ABTS, 2-2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as the substrate of peroxidase and its application to the diagnosis of schistosomiasis. Jpn J Exp Med. 1984 Jun;54(3):131-8. |
ABTS diammonium salt is a substrate for horseradish peroxidase (HRP) conjugate.
A micro-technique of enzyme-linked immunosorbent assay (ELISA) using ABTS, as a substrate for HRP conjugate is studied. In a comparative study among 4 substrates, namely; 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT) and ABTS, for HRP in terms of sensitivity, ABTS is the most sensitive, stable and the best in visuality by its bluish-green color[1]. ABTS is a typical peroxidase substrate. For purification and characterization peroxidase positive transformants are cultivated in large scale (XL) under conditions that yield active protein in the culture supernatant. After 160 h cultivation an activity of 55,000 U/L in relation to the substrate ABTS is achieved and the supernatant containing the peroxidase is harvested. With ABTS as substrate the peroxidase activity falls significantly when the H2O2 concentration rose above 0.125 mM, indicating that the enzyme is inhibited by H2O2. Maximum reaction rates depending upon substrate tested reache values between 31.2 and 125 µM[2].
[1]. Matsuda H, et al. Evaluation of ELISA with ABTS, 2-2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as the substrate of peroxidase and its application to the diagnosis of schistosomiasis. Jpn J Exp Med. 1984 Jun;54(3):131-8. [2]. Lauber C, et al. Identification, heterologous expression and characterization of a dye-decolorizing peroxidase of Pleurotus sapidus. AMB Express. 2017 Aug 23;7(1):164.
Cas No. | 30931-67-0 | SDF | |
Canonical SMILES | CCN1C(C=CC(S(=O)([O-])=O)=C2)=C2S/C1=N/N=C(SC3=C4C=CC(S(=O)([O-])=O)=C3)\N4CC.[NH4+].[NH4+] | ||
Formula | C18H24N6O6S4 | M.Wt | 548.68 |
Solubility | Water : ≥ 50 mg/mL (91.13 mM) | Storage | Store at 2-8°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 1.8226 mL | 9.1128 mL | 18.2256 mL |
5 mM | 0.3645 mL | 1.8226 mL | 3.6451 mL |
10 mM | 0.1823 mL | 0.9113 mL | 1.8226 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5
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