Acridine Orange (Synonyms: NSC 194350) |
Catalog No.GC41242 |
Acridine Orange is a cell-permeable fluorescent dye that stains organisms (bacteria, parasites, viruses, etc.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 494-38-2
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >75.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Acridine orange is a cell-permeable, nucleic acid-selective fluorescent cationic dye useful for cell cycle determination and detection of cellular autophagy. [1][2][3] It exhibits excitation/emission spectra of 502/525, 460/650, and 475/590 nm when bound to dsDNA, bound to ssDNA or RNA, and under acidic conditions, respectively. [1] [2] The ratio of acridine orange emission at 590 to emission at 525 nm can be used to quantify the increase in the number of acidic vesicular organelles observed during cellular autophagy.
Reference:
[1]. Virant-Klun, I., Tomazevic, T., and Meden-Vrtovec, H. Sperm single-stranded DNA, detected by acridine orange staining, reduces fertilization and quality of ICSI-derived embryos. J.Assist.Reprod.Genet. 19(7), 319-328 (2002).
[2]. Han, J., and Burgess, K. Fluorescent indicators for intracellular pH. Chem. Revs. 110(5), 2709-2728 (2010).
[3]. Thomé, M.P., Filippi-Chiela, E.C., Villodre, E.S., et al. Ratiometric analysis of Acridine Orange staining in the study of acidic organelles and autophagy. J. Cell Sci. 129(24), 4622-4632 (2016).
Cas No. | 494-38-2 | SDF | |
Synonyms | NSC 194350 | ||
Chemical Name | N3,N3,N6,N6-tetramethyl-3,6-acridinediamine | ||
Canonical SMILES | CN(C)C1=CC(N=C(C=C(N(C)C)C=C2)C2=C3)=C3C=C1 | ||
Formula | C17H19N3 | M.Wt | 265.4 |
Solubility | 0.3mg/mL in ethanol, 20mg/mL in DMSO, 2mg/mL in DMF | Storage | Store at -20°C, protect from light |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 3.7679 mL | 18.8395 mL | 37.679 mL |
5 mM | 0.7536 mL | 3.7679 mL | 7.5358 mL |
10 mM | 0.3768 mL | 1.8839 mL | 3.7679 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5
(Based on Reviews and 29 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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