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Amidepsine A (Synonyms: FO-2942A)

Catalog No.GC11675

DGAT inhibitor

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Amidepsine A Chemical Structure

Cas No.: 169181-28-6

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Sample solution is provided at 25 µL, 10mM.

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Quality Control & SDS

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Background

Amidepsine A is a fungal metabolite isolated from the culture broth of Humicola sp FO-2942. Amidepsine A is an inhibitor of dylglycerol acyltransferase (DGAT).

Dylglycerol acyltransferase (DGAT) has been involved in catalyzing the formation of triglycerides from diacylglycerol and Acyl-CoA. The reaction is considered the terminal and only committed step in triglyceride synthesis and is essential for the formation of adipose tissue. There are two isozymes of DGAT have been identified: DGAT1 and DGAT2. DGAT-1 deficient mice are lean and resistant to the development of diet-induced obesity or insulin resistance [1]. DGAT2-/- mice demonstrate reduced triglyceride levels and suffer from skin barrier abnormalities [2].

FO-2942 inhibited DGAT activity with an IC50 of 10.2 μM in rat liver microsomes. FO-2942 inhibited triacylglycerol formation in Raji cells with the IC50 value of 15.5 μM [1].

References:
[1] Tomoda H, Tabata N, Ito M, et al.  Amidepsines, inhibitors of diacylglycerol acyltransferase produced by Humicola sp. FO-2942[J]. The Journal of antibiotics, 1995, 48(9): 942-947.
[2] Smith S J, Cases S, Jensen D R, et al.  Obesity resistance and multiple mechanisms of triglyceride synthesis in mice lacking Dgat[J]. Nature genetics, 2000, 25(1): 87-90.
[3] Stone S J, Myers H M, Watkins S M, et al.  Lipopenia and skin barrier abnormalities in DGAT2-deficient mice[J]. Journal of Biological chemistry, 2004, 279(12): 11767-11776.

Chemical Properties

Cas No. 169181-28-6 SDF
Synonyms FO-2942A
Chemical Name 2,4-dimethoxy-6-methyl-benzoic acid, 4-[[4-[[(1-carboxyethyl)amino]carbonyl]-3-hydroxy-5-methylphenoxy]carbonyl]-3-hydroxy-5-methylphenyl ester
Canonical SMILES OC1=C(C(NC(C)C(O)=O)=O)C(C)=CC(OC(C2=C(O)C=C(OC(C3=C(C)C=C(OC)C=C3OC)=O)C=C2C)=O)=C1
Formula C29H29NO11 M.Wt 567.5
Solubility Soluble in methanol Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 1.7621 mL 8.8106 mL 17.6211 mL
5 mM 0.3524 mL 1.7621 mL 3.5242 mL
10 mM 0.1762 mL 0.8811 mL 1.7621 mL
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