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Biotin-tyramide (Synonyms: Biotin-Phenol,Biotin Phenol)

Catalog No.GC13376

Biotin-tyramide is a biotin derivative used for tyramide signal amplification (TSA), as a reagent to amplify both immunohistochemical signals and in situ hybridization protocols.

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Biotin-tyramide Chemical Structure

Cas No.: 41994-02-9

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100mg
$70.00
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1g
$565.00
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Cell experiment [1]:

Tissue

Brain tissue from Long–Evans rats

Preparation Method

Brain tissue from six animals was processed in one run to fluorescently co-label ARs and NeuN, with and without Biotin-tyramide, as well as ARs and GFAP, with and without Biotin-tyramide.

Reaction Conditions

Biotin-tyramide for 30min

Applications

Biotin-tyramide signal amplification (TSA) dramatically increases AR immunoreactivity in the rat brain, including critical regions of the PFC such as the medial PFC (mPFC) and orbitofrontal cortex (OFC). Biotin-tyramide signal amplification is useful for AR detection with both chromogenic and immunofluorescent immunohistochemistry.

References:

[1]. Low KL, Ma C, et,al. Tyramide Signal Amplification Permits Immunohistochemical Analyses of Androgen Receptors in the Rat Prefrontal Cortex. J Histochem Cytochem. 2017 May;65(5):295-308. doi: 10.1369/0022155417694870. Epub 2017 Mar 1. PMID: 28438093; PMCID: PMC5407533.

Background

Biotin-tyramide is used for tyramide signal amplification (TSA) which is a powerful, patented technology that significantly enhances both chromogenic and fluorescent signals. TSA is easily integrated into standard nonradioactive in situ hybridization (ISH) or IHC protocols.

TSA is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to catalyze the deposition of a fluorophore-labeled tyramide amplification reagent onto tissue sections or cell preparation surfaces that have been previously blocked with proteins.

Biotin-tyramide use horseradish peroxidase (HRP) to catalyze covalent deposition of biotin labels directly adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited labels can be detected with streptavidin conjugates for imaging in brightfield or fluorescence microscopy.

Chemical Properties

Cas No. 41994-02-9 SDF
Synonyms Biotin-Phenol,Biotin Phenol
Chemical Name N-(4-hydroxyphenethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide
Canonical SMILES O=C1N[C@@]([H])([C@]([H])(CS2)N1)[C@@H]2CCCCC(NCCC3=CC=C(O)C=C3)=O
Formula C18H25N3O3S M.Wt 363.47
Solubility ≥ 72.6mg/mL in DMSO, ≥ 8.18 mg/mL in EtOH with ultrasonic Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 2.7513 mL 13.7563 mL 27.5126 mL
5 mM 0.5503 mL 2.7513 mL 5.5025 mL
10 mM 0.2751 mL 1.3756 mL 2.7513 mL
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