BSA-Palmitate Saturated Fatty Acid Complex (5 mM) (Synonyms: BSA-PA)

BSA-Palmitate saturated fatty acid complex is composed of palmitic acid and bovine serum albumin (BSA) at an approximately 6:1 molar ratio of palmitate:BSA. It can be used for efficient fatty acid delivery to cells in culture for the purpose of monitoring fatty acid oxidation or similar processes in various cellular metabolic studies.^[1,2,3] The BSA/BSA-FAs are acceptable for use with short-term cell culture applications (acute treatment to 18 hours), however, for long-term applications (25+ hours) the product can/should be filter sterilized using a 0.2 µm filter and sterile receptacle without affecting the performance. For best results, it is recommended that this product be used in conjunction with BSA control for fatty acid complexes (5 mM) (Cat. No. GC46102).

Formulation:5 mM Palmitate:0.8 mM BSA (6:1 palmitate:BSA) in 150 mM sodium chloride, pH 7.4

References:

[1]. Alsabeeh, N., Chausse, B., Kakimoto, P.A., et al. Cell culture models of fatty acid overload: Problems and solutions. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1863(2), 143-151 (2018).

[2]. Wang, D., Green, M.F., McDonnell, E., et al. Oxygen flux analysis to understand the biological function of sirtuins. Methods Mol. Biol. 1077, 241-258 (2013).

[3]. Bentebibel, A., Sebastián, D., Herrero, L., et al. Novel effect of C75 on carnitine palmitoyltransferase I activity and palmitate oxidation. Biochemistry 45(14), 4339-4350 (2006).