|Colcemid Catalog No.GC40664|
Sample solution is provided at 25 µL, 10mM.
|Cas No.||477-30-5||SDF||Download SDF|
|Solubility||30mg/mL in ethanol, 25mg/mL in DMSO or DMF||Storage||Store at -20°C|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Shipping Condition||Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
Colcemid is a colchicine derivative that inhibits tubulin polymerization as potently as colchicine (IC50 = 2.1 and 2.4 μM, respectively) but is less toxic.,, At very low (nanomolar) concentrations, colcemid suppresses microtubule dynamicity and inhibits cell migration, while at micromolar levels it blocks microtubule assembly, arresting cells in metaphase.,, Mitotic block by colcemid is used to synchronize cells and for karyotyping in cytogenetic studies.,6 Prolonged exposure to colcemid can activate p53, leading to apoptosis.
. Muzaffar, A., Brossi, A., Lin, C.M., et al. Antitubulin effects of derivatives of 3-demethylthiocolchicine, methylthio ethers of natural colchicinoids, and thioketones derived from thiocolchicine. Comparison with colchicinoids. J. Med. Chem. 33(2), 567-571 (1990).
. Rieder, C.L., and Palazzo, R.E. Colcemid and the mitotic cycle. Journal of Cell Science 102(3), 387-392 (1992).
. Jordan, M.A., and Wilson, L. Microtubules as a target for anticancer drugs. Nature Reviews.Cancer 4(4), 253-265 (2004).
. Curlej, J., Bulla, J., and Chrenek, P. Occurrence of chromosomal aneuploidy in rabbit oocytes and embryos at different developmental stages. Zygote 18(3), 203-207 (2010).
. Yang, H., Ganguly, A., and Cabral, F. Inhibition of cell migration and cell division correlates with distinct effects of microtubule inhibiting drugs. The Journal of Biological Chemisty 285(42), 32242-32250 (2010).
. Bayani, J., and Squire, J.A. Preparation of cytogenetic specimens from tissue samples. Current Protocols in Cell Biology 22(22.2), 1-15 (2004).
. Sablina, A.A., Chumakov, P.M., Levine, A.J., et al. p53 activation in response to microtubule disruption is mediated by integrin-Erk signaling. Oncogene 20(8), 899-909 (2001).