Digitonin (Synonyms: Digitin, NSC 23471, NSC 237417)

Cell lines

Caco-2 and MCF-7 cells

Preparation Method

The cells were incubated with 100 µl of medium containing different concentrations of digitonin and other secondary metabolites for 24 hours.

Reaction Conditions

0-500µM for 24 hours.

Applications

Digitonin showed the high exhibition to Caco-2, MCF-7 with IC50 of 15.72 ± 0.16 and 5.23 ± 0.61µM, respectively.

Animal models

Inbred rats of both sexes of the Lister-Hooded (LH) strain

Preparation Method

Digitonin was dissolved in dimethylsulfoxide (DMSO) and diluted with saline to yield a solution that contained 400 µM digitonin and 40 µM DMSO; 0.2 ml of the solution was infused into the hepatic artery.

Dosage form

400 µM 0.2 ml of Digitonin solution, a catheter (PE 10, inner diameter 0.28 mm, outer diameter 0.61 mm) was inserted into the hepatic artery proper via the gastroduodenal artery

Applications

After pretreatment with digitonin the platinum content in the tumour as measured at 1 h after CBDCA infusion was 66±19 ng/mg. This was more than 5 times higher than the values obtained after pretreatment with saline alone (12±4 ng/mg) or with DMSO-saline (10±2 ng/mg)

References:

[1]: Eid S Y, El-Readi M Z, Wink M. Digitonin synergistically enhances the cytotoxicity of plant secondary metabolites in cancer cells[J]. Phytomedicine, 2012, 19(14): 1307-1314.
[2]: LindnÉr P G, Heath D, Howell S B, et al. Digitonin enhances the efficacy of carboplatin in liver tumour after intra-arterial administration[J]. Cancer chemotherapy and pharmacology, 1997, 40(5): 444-448.