Home>>Signaling Pathways>> Proteases>> Endogenous Metabolite>>ATP

ATP

Catalog No.GC35420

ATP, as a phosphate-group donor for substrate activation in metabolic reactions.

Products are for research use only. Not for human use. We do not sell to patients.

ATP Chemical Structure

Cas No.: 56-65-5

Size Price Stock Qty
10mM (in 1mL Water)
$52.00
In stock
100mg
$46.00
In stock
500mg
$74.00
In stock

Tel:(909) 407-4943 Email: sales@glpbio.com

Customer Reviews

Based on customer reviews.

  • GlpBio Citations

    GlpBio Citations
  • Bioactive Compounds Premium Provider

    Bioactive Compounds Premium Provider

Sample solution is provided at 25 µL, 10mM.

Product has been cited by 4 publications

Product Documents

Quality Control & SDS

View current batch:

Protocol

Cell experiment [1]:

Cell lines

stem cells from human exfoliated deciduous teeth (SHEDs)

Preparation Method

This study investigated the effects of extracellular ATP at a low (0.1 µM) and high (10 µM) concentration on the stemness and osteogenic differentiation of SHEDs. Cells were cultured in either growth medium or osteogenic medium with or without 0.1-10 µM ATP.

Reaction Conditions

0.1-10 µM ATP;

Applications

In growth medium, both concentrations of ATP increased the mRNA expression of pluripotent and osteogenic markers. In contrast, in osteogenic medium, 0.1 µM ATP enhanced in vitro mineralization, whereas 10 µM ATP inhibited this process.

Animal experiment [2]:

Animal models

rats

Preparation Method

Subcutaneous injection of ATP induces pain-related behavior and hyperalgesia to mechanical and heat stimulation in rats.

Dosage form

100 µM and 1 mM; s.c.

Applications

ATP (100 µM and 1 mM, but not 1 µM) superfused for 5 min before the mechanical stimulation suppressed the mechanical responses of muscle thin fibers irrespective of whether they excited the fiber.

References:

[1] Techatharatip O, et al. Biphasic Effect of ATP on In Vitro Mineralization of Dental Pulp Cells. J Cell Biochem. 2018 Jan;119(1):488-498.
[2] Matsuda T, et al. ATP decreases mechanical sensitivity of muscle thin-fiber afferents in rats. Neurosci Res. 2015 Aug;97:36-44.

Background

ATP, as a phosphate-group donor for substrate activation in metabolic reactions, is required for the biosynthesis of the intracellular second messenger cyclic adenosine monophosphate (cAMP) and mediates intercellular communication as a bona fide extracellular messenger[1]. ATP can promote tumor progression or tumor suppression.[1].

In vitro, the IC50 value for ATP, α,β-meATP, and β,γ-meATP on these P2X receptors regulating IOP (intraocular pressure) was around 1.0 µM. P2X receptors present in the ciliary body are responsible for reducing IOP. Moreover, 1.0 µM ATP under light conditions causes a reduction in IOP of roughly 50% but when darkness occurs, ATP concentration is decreased to 0.30 µM, this concentration scarcely reducing IOP more than 16%[2]. There is a obvious increase in ECM (extracellular matrix) accumulation has been found in AF (annulus fibrosus) cells at a lower ATP treatment level (20 µM) compared with NP (nucleus pulposus) cells (100 µM), suugestng that AF cells are more sensitive to extracellular ATP than NP cells[3].

In vivo test it shown that Wistar rats were treated with 25 mg/kg ATP reduced bevacizumab-induced renal toxicity significantly more effectively than benidipine (4 mg/kg)[4]. Treatment with 1 and 5 mg/kg ATP intra-arterially (i.a.) close to the bladder in rats, produced rapid, phasic, dose-dependent increases in bladder pressure with micturition immediately after injection[5].

Vultaggio-Poma V, et al. Extracellular ATP: A Feasible Target for Cancer Therapy. Cells. 2020 Nov 17;9(11):2496.

Pintor J. Light-induced ATP release from the lens. Purinergic Signal. 2018 Dec;14(4):499-504.

Gonzales S, et al. ATP promotes extracellular matrix biosynthesis of intervertebral disc cells. Cell Tissue Res. 2015 Feb;359(2):635-642.

Kocaturk H, et al. Effect of adenosine triphosphate, benidipine and their combinations on bevacizumab-induced kidney damage in rats. Adv Clin Exp Med. 2021 Nov;30(11):1175-1183.

Igawa Y, et al. Functional importance of cholinergic and purinergic neurotransmission for micturition contraction in the normal, unanaesthetized rat. Br J Pharmacol. 1993 Jun;109(2):473-9.

References:

Chemical Properties

Cas No. 56-65-5 SDF
Canonical SMILES O[C@@H]([C@H]([C@H](N1C=NC2=C1N=CN=C2N)O3)O)[C@H]3COP(O)(OP(OP(O)(O)=O)(O)=O)=O
Formula C10H16N5O13P3 M.Wt 507.18
Solubility Water : ≥ 100 mg/mL (197.17 mM) Storage Store at 2-8°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 1.9717 mL 9.8584 mL 19.7169 mL
5 mM 0.3943 mL 1.9717 mL 3.9434 mL
10 mM 0.1972 mL 0.9858 mL 1.9717 mL
  • Molarity Calculator

  • Dilution Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
**When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / CoA (available online).

Calculate

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Related Video

    ATP- GlpBio

Reviews

Review for ATP

Average Rating: 5 ★★★★★ (Based on Reviews and 27 reference(s) in Google Scholar.)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%
Review for ATP

GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.

Required fields are marked with *

You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.