| Hexa His tag peptide Catalog No.GP10154 |
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
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Purity = 98.00%
- COA (Certificate Of Analysis)
- MS
- HPLC
- SDS (Safety Data Sheet)
- Datasheet
| Cas No. | N/A | SDF | |
| Synonyms | H-His-His-His-His-His-His-OH | ||
| Chemical Name | Hexa His | ||
| Canonical SMILES | C1=C(NN=C1)CC(C(=O)NC(CC2=CC=NN2)C(=O)NC(CC3=CC=NN3)C(=O)NC(CC4=CC=NN4)C(=O)NC(CC5=CC=NN5)C(=O)NC(CC6=CC=NN6)C(=O)O)N | ||
| Formula | C36H44N18O7 | M.Wt | 840.85 |
| Solubility | ≥84.1mg/mL in DMSO, ≥123.4 mg/mL in EtOH with ultrasonic, ≥67.5 mg/mL in H2O | Storage | Desiccate at -20°C |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
| Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
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Hexa His is an amino acid sequence consisting of 6 His residues in a row, it is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Hexa His Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. The form B is active against certain oligosaccharides. The form S has no measurable activity. Hexa His acts as a metal binding site for a recombinant protein. His-Tag sequence can be placed on the N- or -terminal of a target protein by using vectors from various commercial molecular biology companies. And this Tag is often followed by a cleavage site for a specific protease - for example Leu-Val-Arg-Gly-Ser peptide sequence is recognized and cleaved by the protease known as thrombin.
References:
1. Hengen, P (1995). "Purification of His-Tag fusion proteins from Escherichia coli". Trends in Biochemical Sciences 20 (7): 285–6.