Coomassie Blue Fast Staining Solution is a ready-to-use Coomassie Brilliant Blue protein staining solution designed for polyacrylamide gel electrophoresis. It is intended for use by heating in a microwave for 2 minutes and subsequently placing it on a shaker for 5 minutes. This staining solution exhibits extremely high sensitivity, allowing for specific protein staining, with visible bands achievable at protein sample quantities as low as 10 ng. The Coomassie Blue Fast Staining Solution is exceptionally convenient to use, requiring only a one-step staining process, significantly reducing the time needed for staining. It provides a clean background without the need for fixation, washing, or destaining steps, thereby improving the signal-to-noise ratio of the staining process. Furthermore, this formulation is safe, non-toxic, and easy to handle. Its methanol-free composition prevents gel shrinkage.
Fig 1. Proteins of BSA stained with Coomassie Blue Fast Staining Solution on a 10% SDS-PAGE gel, heated for 2 minutes and slowly shake for 5 minutes for effective staining. The first lane is the marker, the second lane contains 1 µg of BSA protein, the third lane contains 100 ng of BSA protein, and the fourth lane contains 10 ng of BSA protein.
References：
[1] Jiuxiao Ruan, Huhui Chen, et al. "Brassinosteroids repress the seed maturation program during the seed-to-seedling transition." Plant Physiol. 2021 May 27;186(1):534-548. PMID: 33620498
[2] Luling He, Qin Gong, et al. "Proteomic changes in rat kidney injured by adenine and the regulation of anemoside B4." Journal of Chinese Pharmaceutical Sciences. January 2019.
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