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Insulin (human) recombinant expressed in yeast

Catalog No.GC18093

Endogenous insulin receptor agonist

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Insulin (human) recombinant expressed in yeast Chemical Structure

Cas No.: 11061-68-0

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10mg
$54.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

IC50: 5.01 nmol/L for insulin receptor

Insulin is a peptide hormone produced by beta cells in pancreas. Insulin has been found to stimulate glucose uptake into muscle and adipose tissue, which is central to the maintenance of whole body glucose homeostasis. Autoimmune destruction of the β-cells in pancreas leads to a lack of insulin production and the development of type I and II diabetes mellitus.

In vitro: Methotrexate (MTX) was found to be linked to insulin covalently. As effectively as insulin, insulin-MTX complex competed with 125I-insulin for insulin receptor. It was found that IC50 and Ki for insulin-MTX were 93.82 nM and 91.88 nM, respectively, whereas those for insulin were 5.01 nM and 4.85 nM, respectively [1].

In vivo: Previous study showed that insulin-stimulated glucose uptake in extensor muscles from SJL mice was reduced, but the basal uptake rates were not different. In another mouse study, knockdown of TC10α but not TC10β in 3T3-L1 adipocytes resulted in a inhibition of both insulin-stimulated glucose uptake and GLUT4 translocation [2].

Clinical trial: Clinical study shows that when insulin levels fails, diabetes mellitus can happen. Thus, insulin is medically used to treat some forms of diabetes mellitus. Biosynthetic human insulin for clinical use is manufactured by recombinant DNA technology. Insulin is often taken as subcutaneous injections . Morevoer, inhaled insulin is currently also available in U.S. market (https://en.wikipedia.org/wiki/Insulin#Medication_uses).

References:
[1] Ou X,Kuang A,Liang Z,Peng X,Zhong Y.  The binding characteristics of insulin-MTX to insulin receptor. Hua Xi Yi Ke Da Xue Xue Bao.2001 Dec;32(4):538-40.
[2] Leney SE,Tavaré JM.  The molecular basis of insulin-stimulated glucose uptake: signalling, trafficking and potential drug targets. J Endocrinol.2009 Oct;203(1):1-18.

Chemical Properties

Cas No. 11061-68-0 SDF
Formula C257H383N65O77S6 M.Wt 5807.57
Solubility <58.08mg/mL in Water Storage -20°C, protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 0.1722 mL 0.8609 mL 1.7219 mL
5 mM 0.0344 mL 0.1722 mL 0.3444 mL
10 mM 0.0172 mL 0.0861 mL 0.1722 mL
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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