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JSH-23 Catalog No.GC11438

NF-κB inhibitor

Size Price Stock Qty
10mM (in 1mL DMSO)
$68.00
In stock
5mg
$57.00
In stock
25mg
$130.00
In stock
500mg
$600.00
In stock
1g
$800.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Quality Control

Quality Control & SDS

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Protocol

Cell experiment [1]:

Cell lines

LPS-stimulated RAW264.7 cells

Preparation method

The solubility of this compound in DMSO is >12mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

0, 1, 3, 10 and 30 μM

Applications

In LPS-stimulated RAW264.7 cells, JSH-23 inhibited LPS-induced SEAP expression in a dose-dependent way by 23±3%, 68±3% and 103±4% at 3 μM, 10 μM and 30 μM, respectively. JSH-23 also dose-dependently decreased LPS-induced DNA binding activity of NF-κB. JSH-23 showed differential inhibitory effects on LPS-induced expressions of the pro-inflammatory transcripts.

Animal experiment [2]:

Animal models

cisplatin-induced acute kidney injury (AKI) male C57BL/6 mice

Dosage form

20 mg/kg (10 mg/kg 8 hours prior to cisplatin injection and 5 mg/kg on days 1 and 2 after cisplatin injection) or 40 mg/kg(20 mg/kg 8 hours prior to cisplatin injection and 20 mg/kg on day 1 after cisplatin injection); intraperitoneal (IP) injection

Application

In cisplatin-induced AKI male C57BL/6 mice, JSH-23 (total dose of 40 mg/kg) significantly reduced BUN, serum creatinine and serum NGAL. JSH-23 resulted in a significant decrease in ATN score and MPO activity but not tubular apoptosis score in the kidney. JSH-23 also significantly decreased IL-1, IL-6, CXCL1 and TNF-α.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1] Shin HM, Kim MH, Kim BH, Jung SH, Kim YS, Park HJ, Hong JT, Min KR, Kim Y. Inhibitory action of novel aromatic diamine compound on lipopolysaccharide-induced nuclear translocation of NF-kappaB without affecting IkappaB degradation. FEBS Lett. 2004 Jul 30;571(1-3):50-4.

[2] Ozkok A1, Ravichandran K1, Wang Q1, et al. NF-κB transcriptional inhibition ameliorates cisplatin-induced acute kidney injury (AKI). Toxicol Lett. 2016 Jan 5;240(1):105-13.

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Chemical Properties

Cas No. 749886-87-1 SDF
Synonyms N/A
Chemical Name 4-methyl-1-N-(3-phenylpropyl)benzene-1,2-diamine
Canonical SMILES CC1=CC(=C(C=C1)NCCCC2=CC=CC=C2)N
Formula C16H20N2 M.Wt 240.34
Solubility ≥24mg/mL in DMSO Storage Store at -20° C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

Background

JSH-23 is an inhibitor of NF-κB transcriptional activity with IC50 value of 7.1μM [1].

JSH-23 is developed to inhibit NF-κB transcriptional activity in LPS-stimulated macrophages RAW 264.7. It shows a dose-dependent inhibition. This effect is not due to its cytotoxicity. In the same condition, JSH-23 is found to significantly decrease the LPS-induced DNA binding activity of NF-κB while decrease nuclear amount of NF-κB p65. JSH-23 plays these roles without affecting IκB degradation. In addition, JSH-23 also shows inhibition effects on the expression of the pro-inflammatory transcripts and enzymes, including IL-6, IL-1β, COX-2 and TNF-α. Furthermore, JSH-23 inhibits LPS-induced apoptotic chromatin condensation [1].

References:
[1] Shin HM, Kim MH, Kim BH, Jung SH, Kim YS, Park HJ, Hong JT, Min KR, Kim Y. Inhibitory action of novel aromatic diamine compound on lipopolysaccharide-induced nuclear translocation of NF-kappaB without affecting IkappaB degradation. FEBS Lett. 2004 Jul 30;571(1-3):50-4.