Laminin (925-933) (Synonyms: CDPGYIGSR, Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg)

Laminin (925-933)（CDPGYIGSR）, is the sequence of Laminin on the B1 chain. YIGSR is active in promoting the adhesion of a variety of epithelial cells^[1]. Laminin binds to colagen IV, heparan sulfate proteoglycan, and nidogen-entactin,and it mediates cellular interactions with this ma-trix^[2].

A laminin-derived synthetic peptide, Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg-NH2 (CDPGYIGSR-H2), containing an active site for cell binding inhibited both angiogenesis and solid tumor growth^[3].

In vitro, at concentrations of 100 and 300 µg/ml, Laminin (925-933) can stimulate the attachment of HT-1080 and CHO cells to culture plates^[2].

In vitro, the oligopeptide domain Laminin (925-933) covalently linked to an agarose gel as a bioartificial 3D substrate successfully supports neurite outgrowth from dorsal root ganglia (DRG)^[2]. In vitro, a 78% reduction in cell attachment was observed on films containing Laminin (925-933) in the cell plating medium^[4].

In vivo experiments revealed the capability of a Laminin (925-933)-derivatized gel to enhance nerve regeneration in a transected rat dorsal root model compared to an underivatized gel, a Laminin (925-933) gel, and saline-filled nerve guidance channels^[3].

References:

[1] Graf J, et al. A pentapeptide from the laminin B1 chain mediates cell adhesion and binds the 67,000 laminin receptor. Biochemistry. 1987 Nov 3;26(22):6896-900.

[2] Graf J, et al. Identification of an amino acid sequence in laminin mediating cell attachment, chemotaxis, and receptor binding. Cell. 1987 Mar 27;48(6):989-96.

[3] Borkenhagen M, et al. Three-dimensional extracellular matrix engineering in the nervous system. J Biomed Mater Res. 1998 Jun 5;40(3):392-400.

[4] Ranieri JP, et al. Spatial control of neuronal cell attachment and differentiation on covalently patterned laminin oligopeptide substrates. Int J Dev Neurosci. 1994 Dec;12(8):725-35.