Propidium iodide (Synonyms: PI) |
Catalog No.GC14228 |
Propidium iodide (PI) is a small red-fluorescent molecule that binds to DNA but cannot passively traverse into cells that possess an intact plasma membrane.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 25535-16-4
Sample solution is provided at 25 µL, 10mM.
Propidium iodide (PI) is a small red-fluorescent molecule that binds to DNA but cannot passively traverse into cells that possess an intact plasma membrane. Propidium iodide uptake versus exclusion can be used to discriminate dead cells. PI is excited by wavelengths between 400 and 600 nm and emits light between 600 and 700 nm [1]. PI intercalates to DNA with no sequence preference with one dye molecule per four to five base pairs [2]. When bound to DNA fluorescence of PI is enhanced 20- to 30-fold [3]. PI binds stoichiometrically to nucleic acids so that fluorescence emission is proportional to the DNA (and RNA, which has to be removed if DNA is to be measured) content of a cell [4].
References:
[1]. Crowley L C, Scott A P, Marfell B J, et al. Measuring cell death by propidium iodide uptake and flow cytometry[J]. Cold Spring Harbor Protocols, 2016, 2016(7): pdb. prot087163.
[2]. Waring M J. Complex formation between ethidium bromide and nucleic acids[J]. Journal of molecular biology, 1965, 13(1): 269-282.
[3]. Arndt-Jovin D J, Jovin T M. Fluorescence labeling and microscopy of DNA[J]. Methods in cell biology, 1989, 30: 417-448.
[4]. Riccardi C, Nicoletti I. Analysis of apoptosis by propidium iodide staining and flow cytometry[J]. Nature protocols, 2006, 1(3): 1458-1461.
Average Rating: 5
(Based on Reviews and 21 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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