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SOTS-1 (technical grade) (Synonyms: Superoxide Thermal Source, Di(4Carboxybenzyl)Hyponitrite)

Catalog No.GC44918

Superoxide radical anion is a toxic by-product of mitochondrial respiration that is formed from approximately 1-4% of the oxygen metabolized by aerobic organisms.

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SOTS-1 (technical grade) Chemical Structure

Cas No.: 223507-96-8

Size Price Stock Qty
500μg
$43.00
In stock
1mg
$60.00
In stock
5mg
$258.00
In stock
10mg
$428.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

Superoxide radical anion is a toxic by-product of mitochondrial respiration that is formed from approximately 1-4% of the oxygen metabolized by aerobic organisms. SOTS-1 is an azo-compound that can be thermally decomposed in aqueous solution to generate superoxide radical anion at a constant, controlled rate. More specifically, SOTS-1 thermally decomposes to form an intermediate that reacts with oxygen at the diffusion-controlled limit to generate superoxide radical anion. The decay of SOTS-1 into the intermediate follows first order kinetics, and exhibits a half-life of 4900 seconds at physiological pH and temperature. This allows for study of the effect of superoxide on biologically relevant systems in a controlled environment that closely mimics conditions in vivo.

Chemical Properties

Cas No. 223507-96-8 SDF
Synonyms Superoxide Thermal Source, Di(4Carboxybenzyl)Hyponitrite
Canonical SMILES O=C(O)C1=CC=C(CON=NOCC2=CC=C(C(O)=O)C=C2)C=C1
Formula C16H14N2O6 M.Wt 330.3
Solubility DMF: 2 mg/ml,DMF:PBS(pH 7.2)(1:2): 0.3 mg/ml,DMSO: 10 mg/ml Storage Store at -80°C, protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 3.0276 mL 15.1378 mL 30.2755 mL
5 mM 0.6055 mL 3.0276 mL 6.0551 mL
10 mM 0.3028 mL 1.5138 mL 3.0276 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Reviews

Review for SOTS-1 (technical grade)

Average Rating: 5 ★★★★★ (Based on Reviews and 12 reference(s) in Google Scholar.)

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