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SYBR Safe DNA Gel Stain Catalog No.GC12792

Safe and sensitive DNA/RNA gel stain

Size Price Stock Qty
500ul
$45.00
In stock
1ml
$66.00
In stock
10*1ml
$384.00
In stock
100*1ml
$2,135.00
In stock

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Tel: (626) 353-8530 Email: sales@glpbio.com

Sample solution is provided at 25 µL, 10mM.

Description

SYBR Safe DNA Gel Stain is a highly sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels. SYBR Safe stain is specifically formulated to be a less hazardous alternative to ethidium bromide (EB) that can be used with either blue-light or UV excitation.

SYBR Safe DNA Gel Stain is a better nucleic acid staining reagent for all of your molecular biology needs. SYBR Safe DNA Gel Stain is not only better for you and the environment, but also your sample and your institution.

SYBR Safe stain is supplied as a concentrate solution that can be used like an ethidium bromide solution. The stain is also suitable for staining RNA in gels.

There is a vast improvement in cloning efficiency of DNA fragments stained with SYBR Safe stain and visualized using blue-light versus the same fragments stained with ethidium bromide and exposed to UV light.

Quality Control

Quality Control & SDS

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Protocol

Staining nucleic acids after electrophoresis

Soak the gel in SYBR Safe stain. If using SYBR Safe gel stain concentrate, dilute 10,000X in TAE or TBE buffer (as appropriate) prior to use. Place the gel in a plastic container, such as a pipet-tip box lid or a household food-storage container. Do not use a glass container, because the dye in the staining solution may adsorb to the walls of the container, resulting in poor gel staining. Add sufficient SYBR™ Safe DNA gel stain to cover the gel. A 50 mL volume is sufficient for staining most standard minigels. To stain larger gels, increase the volume of staining solution in proportion to the increased gel volume, and ensure that the gel is fully immersed during staining.
Incubate for 30 minutes. Cover the gel and the staining solution with aluminum foil or place them in the dark to protect from light. Continuously agitate the gel on an orbital shaker at 50 rpm. No destaining is required.

Precasting SYBR Safe Stain in agarose gels

Prepare the agarose gel directly in SYBR Safe DNA gel stain. SYBR Safe stain is provided in buffer; simply substitute SYBR Safe stain for the buffer when preparing the molten agarose. If using the 10,000X SYBR Safe stain concentrate, dilute the concentrated stain 1:10,000 in agarose gel buffer (e.g., 1X TBE or 1X TAE) and add the buffer plus stain mixture to the powdered agarose. For example if you run TBE gels and require 30 mL of molten agarose for your tray, add 3 μL of 10,000X SYBR Safe stain concentrate to 30 mL of 1X TBE, mix well, and add to the powdered agarose.
Note: You can heat the agarose/SYBR Safe stain mixture in the microwave. As with precasting gels with ethidium bromide, the mobility of nucleic acid fragments in the gel may be somewhat slower when run in these gels compared to their mobility in the gel without stain.
Run the gel. Use a running buffer appropriate to the SYBR Safe gel stain formulation. No post-staining or destaining is needed.

Viewing and photographing the gel

You can view stained gels using a standard 300 nm transilluminator, a 254 nm epi- or transilluminator, or a blue-light transilluminator such as the Safe Imager 2.0 Blue-Light transilluminator. DNA stained with SYBR Safe stain can also be visualized and analyzed using imaging systems equipped with an excitation source in the UV range or between 470–530 nm. 
Note: If bands from the SYBR Safe stained gel are to be excised and used in a ligation reaction, we recommend that the gel is illuminated using blue-light source (i.e., Safe Imager 2.0 Blue-Light transilluminator) and not a UV light source. In some instances, UV light sources in combination with SYBR Safe stain can lead to reduced cloning efficiencies.
You can photograph stained gels using Polaroid 667 black-and-white print film and SYBR Safe photographic filter. SYPRO photographic filter or a Kodak Wratten #9 filter also work well. Using this film and one of these filters, SYBR Safe DNA gel stain provides the same detection sensitivity as ethidium bromide and an appropriate filter. Do not use a standard ethidium bromide photographic filter with SYBR Safe DNA gel stain. Gels stained with SYBR Safe stain can also be imaged using a CCD camera or a laser-based scanner.

SYBR Safe DNA Gel Stain Dilution Calculator

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SYBR Safe DNA Gel Stain Molarity Calculator

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Chemical Properties

Cas No. N/A SDF N/A
Synonyms N/A
Chemical Name N/A
Canonical SMILES N/A
Formula C28H28N2O3S2 M.Wt 504.66
Solubility Soluble in DMSO Storage Store at room temperature in the original container.
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

Features

• Reduce exposure to highly mutagenic EB and harmful UV light.
SYBR Safe DNA Gel Stain is less mutagenic and safer for you to work with than ethidium bromide. You can further decrease your exposure risk by using visible blue-light illumination with SYBR Safe stain rather than UV illumination. This is especially valuable when performing exposure-intensive protocols like cutting bands out of gels.

• Increase sensitivity by reducing nonspecific background fluorescence.
SYBR Safe stain offers excellent sensitivity in nucleic acid visualization with either UV excitation or blue-light excitation. When bound to nucleic acids, the green-fluorescent SYBR Safe stain has fluorescence excitation max at ~280 and ~502 nm, and an emission max at ~530 nm. Plus, when used with blue light illumination, SYBR Safe stain has less background fluorescence than ethidium bromide–stained gels illuminated with UV light.

• Use in place of EB for all staining applications, including RNA staining.
SYBR Safe stain is supplied as a 10,000X concentrate in DMSO which can be used like ethidium bromide solution. SYBR Safe stain can be added into an agarose gel for staining during electrophoresis or the gel can be incubated in SYBR Safe stain solution following electrophoresis. SYBR Safe stain can be stored at room temperature in its original packaging to avoid excessive light exposure.

Background

SYBR Safe DNA Gel Stain is a highly sensitive stain for visualization of DNA in agarose or acrylamide gels. SYBR Safe stain is specifically formulated to be a less hazardous alternative to ethidium bromide that can utilize either blue light or UV excitation.

• Reduce your exposure to highly mutagenic ethidium bromide and harmful UV light
• Increase your sensitivity by reducing nonspecific background fluorescence
• Use in place of ethidium bromide for all staining applications, including RNA staining

A better DNA stain
SYBR Safe DNA Gel Stain is a better nucleic acid staining reagent for all of your molecular biology needs. Not only is SYBR Safe DNA Gel Stain better for you and the environment, it’s better for your sample and your institution. 

Safer than ethidium bromide
SYBR Safe DNA Gel Stain has been specifically formulated and evaluated in a battery of toxicity tests with results indicating so that it is less mutagenic and safer for you to work with than ethidium bromide. An independent laboratory showed reduced mutagenicity of SYBR Safe stain compared to ethidium bromide using the Ames test . You can further decrease your exposure risk by using visible blue-light illumination with SYBR Safe stain rather than UV illumination. This is especially valuable when performing exposure-intensive protocols like cutting bands out of gels.

Excellent sensitivity
SYBR Safe stain offers excellent sensitivity in nucleic acid visualization and documentation applications with either UV excitation or blue-light excitation. When bound to nucleic acids, the green-fluorescent SYBR Safe stain has fluorescence excitation maxima at ~280 and ~502 nm, and an emission maximum at ~530 nm (see figure). Plus, when used with blue light illumination, SYBR® Safe stain has less background fluorescence than ethidium bromide–stained gels illuminated with UV light.

Easy to use
SYBR Safe stain is supplied as a 10,000X concentrate in DMSO which can be used just like a solution of ethidium bromide. SYBR Safe stain can be mixed into an agarose gel for staining during electrophoresis or the gel can be incubated in a solution of SYBR Safe stain following electrophoresis. SYBR Safe stain can be stored at room temperature in its original packaging to avoid excessive light exposure.