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Tetramethylrhodamine methyl ester (perchlorate) Catalog No.GC19476

Non-cytotoxic cell-permeant

Size Price Stock Qty
5mg
$46.80
In stock
10mg
$88.80
In stock
25mg
$164.00
In stock
50mg
$258.00
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Sample solution is provided at 25 µL, 10mM.

Protocol

Cell experiment:

Cultures are exposed to Millipore-filtered solutions (0.22 µm) containing TMRM Perchlorate for 1 hr at 37°C (except the experiment involving different durations of exposure to TMRM Perchlorate). After treatment, solutions are removed and growth media reapplied under sterile conditions, and cultures are post-incubated for 18 hours at 37°C (except for the experiment involving analysis at different time points after exposure). Cells are then stained with 2 mg/mL bisbenzimide for 20 min at room temperature. Coverslips are subsequently washed in saline and imaged using 2P microscopy. Apoptotic cells are identified as brightly fluorescent nuclei under UV excitation indicating DNA fragmentation. Cell survivability is calculated as the percentage of live, unstained cells (±SD) in five microscopic fields per treatment[1].

References:

[1]. Chowdhury SR, et al. Simultaneous evaluation of substrate-dependent oxygen consumption rates and mitochondrial membrane potential by TMRM and safranin in cortical mitochondria. Biosci Rep. 2015 Dec 8;36(1):e00286.
[2]. Monteith A, et al. Imaging of mitochondrial and non-mitochondrial responses in cultured rat hippocampal neurons exposed to micromolar concentrations of TMRM. PLoS One. 2013;8(3):e58059.

Tetramethylrhodamine methyl ester (perchlorate) Dilution Calculator

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Chemical Properties

Cas No. 115532-50-8 SDF Download SDF
Synonyms TMRM, TMR methyl ester
Chemical Name 3,6-bis(dimethylamino)-9-[2-(methoxycarbonyl)phenyl]-xanthylium, perchlorate
Canonical SMILES O=C(OC)C(C=CC=C1)=C1C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4.O=Cl(=O)([O-])=O
Formula C25H25N2O3 • ClO4 M.Wt 500.9
Solubility Soluble in DMSO Storage Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

Background

Tetramethylrhodamine methyl ester (TMRM) (perchlorate) is a non-cytotoxic cell-permeant fluorogenic dye most commonly used to assess mitochondrial function using live cell fluorescence microscopy and flow cytometry.1,2,3 It has two excitation peaks at 515 and 555 nm and an emission peak in the red-orange range (575 nm). Due to the polarization of the mitochondrial membrane, TMRM is taken up into healthy mitochondria. However, when the membrane is depolarized, as in apoptosis, it is not taken up or is released from the mitochondria. Thus, the strength of the fluorescence signal in mitochondria is used to assess cell viability.

Reference: 

1. Farkas, D.L., Wei, M.-d., Febbroriello, P., et al. Simultaneous imaging of cell and mitochondrial membrane potentials Biophys J. 56(6), 1053-1069 (1989).
2. Gandra, P.G., Nogueira, L., and Hogan, M.C. Mitochondrial activation at the onset of contractions in isolated myofibres during successive contractile periods J. Physiol. 590(15), 3597-3609 (2012).
3. Michea, L., Combs, C., Andrews, P., et al. Mitochondrial dysfunction is an early event in high-NaCl-induced apoptosis of mIMCD3 cells Am. J. Physiol. Renal Physiol. 282(6), F981-F990 (2002).