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vitamin D binding protein precursor (353-363) [Homo sapiens] (Synonyms: H2N-Arg-Thr-His-Leu-Pro-Glu-Val-Phe-Leu-Ser-Lys-OH )

Catalog No.GP10001

Highly polymorphic serum glycoprotein

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vitamin D binding protein precursor (353-363) [Homo sapiens] Chemical Structure

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1mg
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5mg
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10mg
$190.00
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25mg
$270.00
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

Human vitamin D-binding protein (hDBP), also known as Gcglobulin, is an abundant, multifunctional, and highly polymorphic serum glycoprotein synthesized by the liver1.  DBP is the major serum transport protein for the vitamin D sterols2, binds and sequesters monomers of actin with high affinity3, 4, and has been identified on the surface of a variety of cell types including B-lymphocytes5, subpopulations of T-lymphocytes6, and the cytotrophoblasts of the placenta7.

DBP is a secreted protein and would therefore be predicted to have a signal sequence. The amino-terminus of the mature serum protein was established by aligning the sequence predicted by the cDNA to a sequenced amino-terminal hDBP peptide8.

References:
1.Haddad, J. G. 1979. Transport of vitamin D metabolites. Clin. Orthop. Relat. Res. 142:249-274.
2.Daiger, S. P., M. S. Schanfield, and L. L. Cavalli-Sforza. 1975. Group specific component (Gc) proteins bind vitamin D and 25-hydroxyvitamin D. Proc. Natl. Acad. Sci. USA. 72:2076-2080.
3.Cooke, N. E., J. Walgate, and J. G. Haddad. 1979. Human serum binding protein for vitamin D and its metabolites. I. Physicochemical and immunological identification in human tissues. J. Biol. Chem. 254: 5965-5971.
4.Van Baelen, H., R. Bouillon, and P. DeMoor. 1980. Vitamin D binding protein (Gc-Globulin) binds actin. J. Biol. Chem. 255:2270-2272.
5.Petrini, M., D. L. Emerson, and R. M. Galbraith. 1983. Linkage between surface immunoglobulin and cytoskeleton of B lymphocytes may involve Gc protein. Nature (Lond.). 306:73-74.
6.Petrini, M., R. M. Galbraith, D. L. Emerson, A. E. Nel, and P. Arnaud. 1985. Structural studies of T lymphocyte Fc receptors. J. Biol. Chem. 260:1804-1810.
7.Nestler, J. E., E. Lange, J. McLeod, H. J. Kliman, and J. F. Strauss. 1985. Detection of vitamin D binding protein (DBP) in human cytotrophoblasts. Seventh Annual American Societyfor Bone and Mineral Research. Wash. DC. 266a. (Abstr.).
8.Svasti, J., A. Kurosky, A. Bennett, and B. H. Bowman. 1979. Molecular basis for the three major forms of human serum vitamin D binding protein (group specific component). Biochemistry. 18:1611-1617.

Chemical Properties

Cas No. SDF
Synonyms H2N-Arg-Thr-His-Leu-Pro-Glu-Val-Phe-Leu-Ser-Lys-OH
Canonical SMILES NC(CCCNC(N)=N)C(NC(C(C)O)C(NC(CC1=CN=CN1)C(NC(CC(C)C)C(N(CCC2)C2C(NC(CCC(O)=O)C(NC(C(C)C)C(NC(CC3=CC=CC=C3)C(NC(CC(C)C)C(NC(CO)C(NC(CCCCN)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O
Formula C61H99N17O16 M.Wt 1326.54
Solubility ≥132.7mg/mL in DMSO Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 0.7538 mL 3.7692 mL 7.5384 mL
5 mM 0.1508 mL 0.7538 mL 1.5077 mL
10 mM 0.0754 mL 0.3769 mL 0.7538 mL
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