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X-NeuNAc

Catalog No.GC16527

A chromogenic substrate for neuraminidase

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X-NeuNAc Chemical Structure

Cas No.: 160369-85-7

Size Price Stock Qty
10mM (in 1mL DMSO)
$45.00
In stock
10mg
$54.00
In stock
50mg
$175.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

Km: 0.89 mM for neuraminidase

X-Neu5Ac is a new substrate for chromogenic assay of neuraminidase activity in bacterial expression systems.

Many studies have focused on the functional role of cell surface gangliosides, which are thought to facilitate antigenicity, cell-cell recognition and cell growth regulation. Nacetyl neuraminic acid (Neu5Ac) within gangliosides Plays a key function in these roles. Chromogenic substrates, such as Neu5Ac, are used routinely for cytologic, histologic and spectroscopic analyses of enzyme activity.

In vitro: X-Neu5Ac was hydrolyzed by neuraminidase to release a halogenated product undergoing rapid aerobic oxidation to form the dark blue pigment. Preliminary kinetic studies indicated that X-Neu5Ac was a good and stable substrate for neuraminidase. In addition, X-Neu5Ac would also be hydrolyzed mutant enzymes [1].

In vivo: To visualize extracellular sialidase activity on the membrane surface in the rat brain, acute brain slices were incubated with X-Neu5Ac at pH 7.3. After 1h, myelin-abundant regions showed intense fluorescence in the rat brain. Although the hippocampus showed weak fluorescence in the brain, mossy fiber terminals in the hippocampus showed relatively intense fluorescence. In addition, the fluorescence intensities caused by X-Neu5Ac was correlated with the sialidase activity. Therefore, staining with X-Neu5Ac was specific for sialidase and useful for quantitative analysis of sialidase activities [1].

Clinical trial: N/A

References:
[1] Fujii I,Iwabuchi Y,Teshima T,Shiba T,Kikuchi M.  X-Neu5Ac: a novel substrate for chromogenic assay of neuraminidase activity in bacterial expression systems. Bioorg Med Chem.1993 Aug;1(2):147-9.
[2] Minami A,Shimizu H,Meguro Y,Shibata N,Kanazawa H,Ikeda K,Suzuki T.  Imaging of sialidase activity in rat brain sections by a highly sensitive fluorescent histochemical method. Neuroimage.2011 Sep 1;58(1):34-40.

Chemical Properties

Cas No. 160369-85-7 SDF
Chemical Name sodium;(2S,4S,6R)-5-acetamido-2-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-4-hydroxy-6-[(1S,2S)-1,2,3-trihydroxypropyl]oxane-2-carboxylate
Canonical SMILES CC(=O)NC1C(CC(OC1C(C(CO)O)O)(C(=O)[O-])OC2=CNC3=C2C(=C(C=C3)Br)Cl)O.[Na+]
Formula C19H21BrClN2NaO9 M.Wt 559.72
Solubility ≥ 28mg/mL in DMSO Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 1.7866 mL 8.933 mL 17.8661 mL
5 mM 0.3573 mL 1.7866 mL 3.5732 mL
10 mM 0.1787 mL 0.8933 mL 1.7866 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 30 reference(s) in Google Scholar.)

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