1,1’-Ethylidene-bis-(L-tryptophan) (Synonyms: Peak 97 Contaminant, Peak E Contaminant, Peak E Substance) |
| Catalog No.GC49768 |
A potential impurity found in commercial preparations of L-tryptophan
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 132685-02-0
Sample solution is provided at 25 µL, 10mM.
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1,1’-Ethylidene-bis-(L-tryptophan) is a potential impurity found in commercial preparations of L-tryptophan.1 It increases the proliferation of EoL-3 eosinophilic leukemia cells, release of eosinophil cationic protein from isolated human peripheral blood eosinophils, and IL-5 production in isolated human T cells.2 1,1’-Ethylidene-bis-(L-tryptophan) (40 µg/kg) induces fascia thickening and increases mast cell infiltration, inflammation, and fibrosis in superficial adipose and panniculus carnosus tissues in mice as a model system of eosinophilia-myalgia syndrome.3
1.Trucksess, M.W.Separation and isolation of trace impurities in L-tryptophan by high-performance liquid chromatographyJ. Chromatogr.630(1-2)147-150(1993) 2.Yamaoka, K.A., Miyasaka, N., Inuo, G., et al.1,1’-Ethylidenebis(tryptophan) (Peak E) induces functional activation of human eosinophils and interleukin 5 production from T lymphocytes: Association of eosinophilia-myalgia syndrome with a L-tryptophan contaminantJ. Clin. Immunol.14(1)50-60(1994) 3.Love, L.A., Rader, J.I., Crofford, L.J., et al.Pathological and immunological effects of ingesting L-tryptophan and 1,1’-ethylidenebis (L-tryptophan) in Lewis ratsJ. Clin. Invest.91(3)804-811(1993)
| Cas No. | 132685-02-0 | SDF | |
| Synonyms | Peak 97 Contaminant, Peak E Contaminant, Peak E Substance | ||
| Canonical SMILES | CC(N1C2=CC=CC=C2C(C[C@H](N)C(O)=O)=C1)N3C4=CC=CC=C4C(C[C@H](N)C(O)=O)=C3 | ||
| Formula | C24H26N4O4 | M.Wt | 434.5 |
| Solubility | 1 M NaOH: soluble | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.3015 mL | 11.5075 mL | 23.015 mL |
| 5 mM | 0.4603 mL | 2.3015 mL | 4.603 mL |
| 10 mM | 0.2301 mL | 1.1507 mL | 2.3015 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 2 reference(s) in Google Scholar.)
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