Home>>Lipids>> P450>>(±)8(9)-EET-d11

(±)8(9)-EET-d11 (Synonyms: (±)8,9-EET-d11, (±)8,9-EpETrE-d11)

Catalog No.GC46264

A neuropeptide with diverse biological activities

Products are for research use only. Not for human use. We do not sell to patients.

(±)8(9)-EET-d11 Chemical Structure

Cas No.: N/A

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10 μg
$168.00
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25 μg
$399.00
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50 μg
$756.00
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Sample solution is provided at 25 µL, 10mM.

Description of (±)8(9)-EET-d11

(±)8(9)-EET-d11 contains 11 deuterium atoms at the 16, 16', 17, 17', 18, 18', 19, 19', 20, 20, and 20 positions. It is intended for use as an internal standard for the quantification of (±)8(9)-EET by GC- or LC-mass spectrometry (MS). (±)8(9)-EET is biosynthesized from arachidonic acid in rat and rabbit liver microsomes by CYP450.1,2 (±)8(9)-EET is a major P450 metabolite in the renal cortex.3 (±)8(9)-EET reduces GFR through cyclooxygenase-dependent preglomerular vasoconstriction.4

1.Chacos, N., Falck, J.R., Wixtrom, C., et al.Novel epoxides formed during the liver cytochrome P-450 oxidation of arachidonic acidBiochem. Biophys. Res. Commun.104(3)916-922(1982) 2.Oliw, E.H., Guengerich, F.P., and Oates, J.A.Oxygenation of arachidonic acid by hepatic monooxygenases. Isolation and metabolism of four epoxide intermediatesJ. Biol. Chem.257(7)3771-3781(1982) 3.Zhang, J.Y., Prakash, C., Yamashita, K., et al.Regiospecific and enantioselective metabolism of 8,9-epoxyeicosatrienoic acid by cyclooxygenaseBiochem. Biophys. Res. Commun.183(1)138-143(1992) 4.Katoh, T., Takahashi, K., Capdevila, J., et al.Glomerular stereospecific synthesis and hemodynamic actions of 8,9-epoxyeicosatrienoic acid in rat kidneyAm. J. Physiol.261(4 Pt 2)F578-F586(1991)

Chemical Properties of (±)8(9)-EET-d11

Cas No. N/A SDF
Synonyms (±)8,9-EET-d11, (±)8,9-EpETrE-d11
Canonical SMILES [2H]C(C([2H])([2H])C([2H])([2H])C([2H])([2H])[2H])([2H])C([2H])([2H])/C=C\C/C=C\C[C@@H](O1)[C@@H]1C/C=C\CCCC(O)=O
Formula C20H21D11O3 M.Wt 331.5
Solubility DMF: 50 mg/ml,DMSO: 50 mg/ml,Ethanol: 50 mg/ml,PBS (pH 7.2): 1 mg/ml Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of (±)8(9)-EET-d11

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1 mg 5 mg 10 mg
1 mM 3.0166 mL 15.083 mL 30.1659 mL
5 mM 0.6033 mL 3.0166 mL 6.0332 mL
10 mM 0.3017 mL 1.5083 mL 3.0166 mL
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In vivo Formulation Calculator (Clear solution) of (±)8(9)-EET-d11

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 28 reference(s) in Google Scholar.)

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