22(R)-hydroxy Cholesterol (Synonyms: Narthesterol) |
| Catalog No.GC14972 |
22(R)-hydroxy Cholesterol is an endogenous agonist of the liver X receptor (LXR), with an EC50 value of 325nM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 17954-98-2
Sample solution is provided at 25 µL, 10mM.
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22(R)-hydroxy Cholesterol is an endogenous agonist of the liver X receptor (LXR), with an EC50 value of 325nM [1]. The LXRs (LXRα and LXRβ) can regulate the expression of cholesterol 7α-hydroxylase induced by oxidosteroids, and cholesterol 7α-hydroxylase is the rate-limiting enzyme in the classical bile acid synthesis process [2]. 22(R)-hydroxy Cholesterol can be used to induce cell differentiation and protect neuronal cells [3-4].
In vitro, 22(R)-hydroxy Cholesterol (30nM-3μM; 96h) treatment significantly inhibited the proliferation of LNCaP prostate cancer cells in a dose-dependent manner [5]. 22(R)-hydroxy Cholesterol (2μM; 14 days) treatment induced hMSCs cells to exhibit neuronal cell-like characteristics, increasing the average cell area, the length of neurites and axons, and increasing the expression of dopamine neuron cell-specific proteins and the percentage of MAP2-positive cells [6].
References:
[1] Spencer, T.A., Dansu, L., Russel, J.S., et al. Pharmacophore analysis of the nuclear oxysterol receptor LXRα. J. Med. Chem. 44(6), 886-897 (2001).
[2] Repa, J.J., Turley, S.D., Lobaccaro, J.-M.A., et al. Regulation of absorption and ABC1-mediated efflux of cholesterol by RXR heterodimers. Science 289(5484), 1524-1529 (2000).
[3] Yao Z X, Han Z, Xu J, et al. 22R-Hydroxycholesterol induces differentiation of human NT2 precursor (Ntera2/D1 teratocarcinoma) cells[J]. Neuroscience, 2007, 148(2): 441-453.
[4] Yao Z X, Brown R C, Teper G, et al. 22R‐Hydroxycholesterol protects neuronal cells from β‐amyloid‐induced cytotoxicity by binding to β‐amyloid peptide[J]. Journal of neurochemistry, 2002, 83(5): 1110-1119.
[5] Chuu C P, Lin H P. Antiproliferative effect of LXR agonists T0901317 and 22 (R)-hydroxycholesterol on multiple human cancer cell lines[J]. Anticancer research, 2010, 30(9): 3643-3648.
[6] Singh M, Jain M, Bose S, et al. 22 (R)-hydroxycholesterol for dopaminergic neuronal specification of MSCs and amelioration of Parkinsonian symptoms in rats[J]. Cell death discovery, 2021, 7(1): 13.
| Cell experiment [1]: | |
Cell lines | Human mesenchymal stem cells (hMSCs) |
Preparation Method | For neuronal differentiation, induction medium containing neurobasal media, B27 supplement, EGF, FGF2 (10ng/mL each) and 22(R)-hydroxy Cholesterol (2µM), l-glutamine, PenStrep was used. The induction protocol was carried out for 14 days with media change on every 3rd day. After the 14-day induction period ended, these cells were then used for subsequent immunocytochemical analysis. |
Reaction Conditions | 2μM; 14 days |
Applications | 22(R)-hydroxy Cholesterol induction significantly increased the expression of dopamine neuron-specific proteins in hMSCs cells and the percentage of MAP2-positive cells. |
References: | |
| Cas No. | 17954-98-2 | SDF | |
| Synonyms | Narthesterol | ||
| Chemical Name | cholest-5-ene-3β,22R-diol | ||
| Canonical SMILES | CC(C)CC[C@@H](O)[C@@H](C)C1CCC2C3CC=C4C[C@@H](O)CC[C@]4(C)C3CC[C@@]21C | ||
| Formula | C27H46O2 | M.Wt | 402.7 |
| Solubility | ≤20mg/ml in ethanol;0.1mg/ml in DMSO;2mg/ml in dimethyl formamide | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.4832 mL | 12.4162 mL | 24.8324 mL |
| 5 mM | 496.6 μL | 2.4832 mL | 4.9665 mL |
| 10 mM | 248.3 μL | 1.2416 mL | 2.4832 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 14 reference(s) in Google Scholar.)
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