3,3',5'-Triiodo-L-thyronine (Synonyms: Reverse T3,rT3,3,3’,5’-T3) |
| Catalog No.GC10384 |
3,3',5'-Triiodo-L-thyronine is a thyroid hormone generated by deiodination of the prohormone thyroxine.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 5817-39-0
Sample solution is provided at 25 µL, 10mM.
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3,3',5'-triiodo-L-thyronine (reverse T3 or rT3) and 3,5,3'-triiodo-L-thyronine (T3) are the metabolism of thyroxine (T4) [1]. 3,3',5'-Triiodo-L-thyronine is thyroid hormone receptors TRα and TRβ antagonist [1].
Thyroid hormones play important roles in the development of the mammalian brain by acting on migration and differentiation of neural cells, synaptogenesis, and myelination. The actions of thyroid hormones are mediated by nuclear thyroid hormone receptors (TRs) and regulation of gene expression. TRs have been also involved in adult brain function. In adult mice, TRα1 deletion and a dominant negative mutant receptor expression induce consistent behavioral changes leading to severe anxiety and morphological changes in the hippocampus [2].
The rT3 was about 1,000-fold less active at the thyroid hormone receptors TRα and TRβ compared with 3,3',5-triiodo-L-thyronine and commonly described as inactive [1].It has been reported that rT3 activates a native form of TRα, TRΔα1, which lacks a nuclear localization signal and functions in the cytoplasm. Through this action, rT3 initiates actin polymerization, particularly in astrocytes and neurons [3].
References:
[1] Nicod P, Burger A, Staeheli V, et al. A radioimmunoassay for 3, 3′, 5′-triiodo-L-thyronine in unextracted serum: method and clinical results[J]. The Journal of Clinical Endocrinology & Metabolism, 1976, 42(5): 823-829.
[2] Bernal J. Thyroid hormone receptors in brain development and function[J]. Nature clinical practice Endocrinology & metabolism, 2007, 3(3): 249-259.
[3] Senese R, Cioffi F, De Lange P, et al. Thyroid: biological actions of ‘nonclassical’thyroid hormones[J]. Journal of Endocrinology, 2014, 221(2): R1-R12.
| Cas No. | 5817-39-0 | SDF | |
| Synonyms | Reverse T3,rT3,3,3’,5’-T3 | ||
| Chemical Name | 2-amino-3-(4-(4-hydroxy-3,5-diiodophenoxy)-3-iodophenyl)propanoic acid | ||
| Canonical SMILES | O=C(O)C(N)CC1=CC=C(OC2=CC(I)=C(O)C(I)=C2)C(I)=C1 | ||
| Formula | C15H12I3NO4 | M.Wt | 651 |
| Solubility | ≥ 32.55mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.5361 mL | 7.6805 mL | 15.361 mL |
| 5 mM | 0.3072 mL | 1.5361 mL | 3.0722 mL |
| 10 mM | 0.1536 mL | 0.768 mL | 1.5361 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 20 reference(s) in Google Scholar.)
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