Δ4-Dafachronic Acid (Synonyms: 3-keto-4-Cholestenoic Acid) |
| Catalog No.GC17515 |
DAF-12 activator
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 23017-97-2
Sample solution is provided at 25 µL, 10mM.
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Δ4-Dafachronic acid is a DAF-12 activator.
Steroid hormone dafachronic acid (DA) has been identified to regulate dauer formation and lifespan in Caenorhabditis elegans via binding to the nuclear receptor DAF-12. DAF-12, an orphan nuclear hormone receptor, regulates dauer larva diapause, reproductive development, fat metabolism, as well as life cycle/longevity in C. elegans. In addition, DAF-12 is needed for entry into dauer by acting as a transcriptional repressor of target genes that favor reproductive development when it is not bound to ligand.
In vitro: Δ4-Dafachronic acid is a racemic version of a sterol-derived hormone acting as a ligand of DAF-12. In a previous study, it was found that there was no significant increase in the Δ4- and Δ4-dafachronic acid concentration in the long-lived glp-1 mutant, nor in the mutants defective in dauer formation [1]. Another previous study provided insights into the molecular interface between DA and IIS pathways and suggested that Δ4- and Δ7-dafachronic acid pathways had unique as well as overlapping biological functions in the control of development and lifespan [2].
In vivo: Up to now, there is no animal in vivo study reported for Δ4-Dafachronic acid.
Clinical trial: So far, no clinical study has been conducted.
References:
[1] Li TM et al. No Significant Increase in the Δ4- and Δ7-Dafachronic Acid Concentration in the Long-Lived glp-1 Mutant, nor in the Mutants Defective in Dauer Formation. G3 (Bethesda). 2015 May 12;5(7):1473-9.
[2] Dumas, K. J.,Guo, C.,Wang, X., et al. Functional divergence of dafachronic acid pathways in the control of C. elegans development and lifespan. Dev.Biol. 340(2), 605-612 (2010).
| Cas No. | 23017-97-2 | SDF | |
| Synonyms | 3-keto-4-Cholestenoic Acid | ||
| Chemical Name | 3-oxo-cholest-4-en-26-oic acid | ||
| Canonical SMILES | O=C1CC[C@@]2(C)C(CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@@H]4[C@@H](CCCC(C)C(O)=O)C)=C1 | ||
| Formula | C27H42O3 | M.Wt | 414.6 |
| Solubility | ≤12.5mg/ml in ethanol;2.5mg/ml in DMSO;5mg/ml in dimethyl formamide | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.412 mL | 12.0598 mL | 24.1196 mL |
| 5 mM | 0.4824 mL | 2.412 mL | 4.8239 mL |
| 10 mM | 0.2412 mL | 1.206 mL | 2.412 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 29 reference(s) in Google Scholar.)
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