Home>>Natural Products>>5(6)-FAM SE

5(6)-FAM SE (Synonyms: 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester; 5(6)-Carboxyfluorescein succinimidyl ester mixed isomers; FITC-NHS)

Catalog No.GC33461

5(6)-FAM SE is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.

Products are for research use only. Not for human use. We do not sell to patients.

5(6)-FAM SE Chemical Structure

Cas No.: 117548-22-8

Size Price Stock Qty
10mM (in 1mL DMSO)
$26.00
In stock
50mg
$54.00
In stock
100mg
$79.00
In stock

Tel:(909) 407-4943 Email: sales@glpbio.com

Customer Reviews

Based on customer reviews.

  • GlpBio Citations

    GlpBio Citations
  • Bioactive Compounds Premium Provider

    Bioactive Compounds Premium Provider

Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

View current batch:

Background

5(6)-FAM SE is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.

[1]. Stafford JM, et al. Accessory factors facilitate the binding of glucocorticoid receptor to the phosphoenolpyruvate carboxykinase gene promoter. J Biol Chem. 2001 Oct 26;276(43):39885-91. [2]. Powell RD, et al. Combined fluorescent and gold immunoprobes: reagents and methods for correlative light and electron microscopy. Microsc Res Tech. 1998 Jul 1;42(1):2-12.

Chemical Properties

Cas No. 117548-22-8 SDF
Synonyms 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester; 5(6)-Carboxyfluorescein succinimidyl ester mixed isomers; FITC-NHS
Canonical SMILES OC1=CC=C2C(OC(C(C=C3)=C2C(C=CC(C(ON(C(CC4)=O)C4=O)=O)=C5)=C5C(O)=O)=CC3=O)=C1.OC6=CC=C7C(OC(C(C=C8)=C7C(C=C(C(ON(C(CC9)=O)C9=O)=O)C=C%10)=C%10C(O)=O)=CC8=O)=C6
Formula C25H15NO9 M.Wt 473.39
Solubility 83.33 mg/mL in DMSO (ultrasonic and warming and heat to 50°C) Storage Store at -20°C, protect from light, stored under nitrogen
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 2.1124 mL 10.5621 mL 21.1242 mL
5 mM 0.4225 mL 2.1124 mL 4.2248 mL
10 mM 0.2112 mL 1.0562 mL 2.1124 mL
  • Molarity Calculator

  • Dilution Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
**When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / CoA (available online).

Calculate

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Reviews

Review for 5(6)-FAM SE

Average Rating: 5 ★★★★★ (Based on Reviews and 1 reference(s) in Google Scholar.)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%
Review for 5(6)-FAM SE

GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.

Required fields are marked with *

You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.