6,6′-Trehalose Dioleate (Synonyms: 6,6′-TDO) |
| Catalog No.GC92071 |
6,6′-Trehalose dioleate (6,6′-TDO) is a glycolipid that has been used in the generation of lipid nanoparticles (LNPs) for the delivery of mRNA in vitro and in vivo.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 338733-40-7
Sample solution is provided at 25 µL, 10mM.
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6,6′-Trehalose dioleate (6,6′-TDO) is a glycolipid that has been used in the generation of lipid nanoparticles (LNPs) for the delivery of mRNA in vitro and in vivo.1 LNPs containing 6,6′-TDO and encapsulating mRNA encoding influenza A hemagglutinin A (HA) increase Ifn-γ, Il-2, and Tnf-α secretion by isolated mouse splenocytes. LNPs containing 6,6′-TDO accumulate in the liver and spleen after intramuscular administration in mice. Unlike LNP formulations that do not contain 6,6′-TDO, LNPs containing 6,6′-TDO do not decrease serum levels of blood urea nitrogen (BUN) or increase serum levels of lactate dehydrogenase (LDH), troponin I, alanine transaminase (ALT), and aspartate aminotransferase (AST) in mice. LNPs containing 6,6′-TDO and encapsulating mRNA encoding influenza A HA increase serum IgG1 and IgG2a titers in mice.
References:
[1]. Bae, S.-H., Yoo, S., Lee, J., et al.A lipid nanoparticle platform incorporating trehalose glycolipid for exceptional mRNA vaccine safetyBioact. Mater.38486-498(2024).
| Cas No. | 338733-40-7 | SDF | |
| Synonyms | 6,6′-TDO | ||
| Chemical Name | (9Z)-9-octadecenoate, 6-O-[(9Z)-1-oxo-9-octadecen-1-yl]-α-D-glucopyranosyl, α-D-glucopyranoside | ||
| Canonical SMILES | O[C@H]([C@H]1O)[C@H](O[C@@H]([C@H]1O)COC(CCCCCCC/C=C\CCCCCCCC)=O)O[C@@H]2[C@H](O)[C@@H](O)[C@H](O)[C@@H](COC(CCCCCCC/C=C\CCCCCCCC)=O)O2 | ||
| Formula | C48H86O13 | M.Wt | 871.2 |
| Solubility | Chloroform: Slightly Soluble: 0.1-1 mg/ml,Methanol: Slightly Soluble: 0.1-1 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.1478 mL | 5.7392 mL | 11.4784 mL |
| 5 mM | 0.2296 mL | 1.1478 mL | 2.2957 mL |
| 10 mM | 0.1148 mL | 0.5739 mL | 1.1478 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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