Ac-IETD-CHO (trifluoroacetate salt) (Synonyms: Caspase-8 Inhibitor)

Ac-IETD-CHO is an inhibitor of caspase-8 (IC50 = 5 nM). [1]  It inhibits fluticasone propionate-induced increases in apoptosis in peripheral blood T-cells isolated from patients with asthma when used at a concentration 60 μM.[2]  Ac-IETD-CHO (10 mg/kg) inhibits increases in hepatic caspase-3 activity, DNA fragmentation, cytosolic cytochrome c levels, and proteolytic processing of caspase-3, -8, and -9 proenzymes in a mouse model of Fas-induced apoptosis and liver failure.[3]

Reference:
[1]. Smith, G.K., Barrett, D.G., Blackburn, K.J., et al. Expression, preparation, and high-throughput screening of caspase-8: Discovery of redox-based and steroid diacid inhibition. Arch. Biochem. Biophys. 399(2), 195-205 (2002).
[2]. Pace, E., Gagliardo, R., Melis, M., et al. Synergistic effects of fluticasone propionate and salmeterol on in vitro T-cell activation and apoptosis in asthma. J. Allergy. Clin. Immunol. 114(5), 1216-1223 (2004).
[3]. Bajt, M.L., Lawson, J.A., Vonderfecht, S.L., et al. Protection against Fas receptor-mediated apoptosis in hepatocytes and nonparenchymal cells by a caspase-8 inhibitor in vivo: Evidence for a postmitochondrial processing of caspase-8. Toxicol. Sci. 58(1), 109-117 (2000).