Alloisolithocholic Acid-d4 (Synonyms: AILCA-d4; AlloisoLCA-d4; β-hydroxy-5-Cholenoic Acid-d4; IsoalloLCA-d4; Isoallolithocholic Acid-d4) |
| Catalog No.GC91985 |
Alloisolithocholic acid-d4 is intended for use as an internal standard for the quantification of alloisolithocholic acid by GC- or LC-MS.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2410277-60-8
Sample solution is provided at 25 µL, 10mM.
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Alloisolithocholic acid-d4 is intended for use as an internal standard for the quantification of alloisolithocholic acid by GC- or LC-MS. Alloisolithocholic acid is a metabolite of the secondary bile acid metabolite 3-oxo lithocholic acid (dehydrolithocholic acid).1 It is formed from 3-oxo lithocholic acid by gut microbiota.1 Alloisolithocholic acid (20 ?M) increases the association of the nuclear hormone receptor nerve growth factor IB (Nr4a1) to the gene encoding forkhead box protein P3 (Foxp3) in isolated mouse CD4+ T cells. It induces the differentiation of isolated mouse T cells into regulatory T cells (Tregs) and the production of mitochondrial reactive oxygen species (ROS) in isolated mouse CD4+ T cells when used at a concentration of 20 ?M.2 Alloisolithocholic acid (6 mg/kg) reduces hepatic bile flow and bile salt, cholesterol, and phospholipid secretion, as well as induces loss of microvilli and dilation of canaliculi in hepatocytes, markers of cholestasis, in rats.3 Fecal levels of alloisolithocholic acid are reduced in patients with Crohn’s disease or ulcerative colitis.1
References:
[1]. Li, W., Hang, S., Fang, Y., et al.A bacterial bile acid metabolite modulates Treg activity through the nuclear hormone receptor NR4A1Cell Host Microbe29(9)1366-137.
[7]. e1369(2021).
[2]. Hang, S., Paik, D., Yao, L., et al.Bile acid metabolites control TH17 and Treg cell differentiationNature576(7785)143-148(2019).
[3]. Vonk, R.J., Tuchweber, B., Massé, D., et al.Intrahepatic cholestasis induced by allo monohydroxy bile acid in ratsGastroenterology80(2)242-249(1981).
| Cas No. | 2410277-60-8 | SDF | |
| Synonyms | AILCA-d4; AlloisoLCA-d4; β-hydroxy-5-Cholenoic Acid-d4; IsoalloLCA-d4; Isoallolithocholic Acid-d4 | ||
| Chemical Name | (5α)-3β-hydroxy-d4-cholan-24-oic acid | ||
| Canonical SMILES | C[C@H](CCC(O)=O)[C@@]1([H])CC[C@@]2([H])[C@]3([H])CC[C@@]4([H])C([2H])([2H])[C@@H](O)C([2H])([2H])C[C@]4(C)[C@@]3([H])CC[C@@]21C | ||
| Formula | C24H36D4O3 | M.Wt | 380.6 |
| Solubility | DMSO: Slightly soluble: 0.1-1 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.6274 mL | 13.1372 mL | 26.2743 mL |
| 5 mM | 0.5255 mL | 2.6274 mL | 5.2549 mL |
| 10 mM | 0.2627 mL | 1.3137 mL | 2.6274 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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