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Amifloxacin (Win49375)

Catalog No.GC33997

Amifloxacin (Win49375) (Win49375) is a synthetic antibacterial agent of the quinolone class.

Products are for research use only. Not for human use. We do not sell to patients.

Amifloxacin (Win49375) Chemical Structure

Cas No.: 86393-37-5

Size Price Stock Qty
1mg
$495.00
In stock
5mg
$675.00
In stock
10mg
$990.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Animal experiment [1]:

Animal models

ICR female mice weighing 18 to 20 g

Preparation Method

ICR female mice were inoculated intraperitoneally with 0.5 ml of the E. coli Vogel bacterial suspension and then distributed into subgroups of 10 to receive serial twofold doses of the test agents and a subgroup of 20 to serve as untreated controls. The infected mice were medicated p.o. 0.8-1.3 mg/kg or s.c. 0.2-1.6 mg/kg of Amifloxacin (Win49375). The number of survivors was determined daily for 7 days.

Dosage form

0.8-1.3 mg/kg/d for 7 days, p.o.; 0.2-1.6 mg/kg/d for 7 days, s.c.

Applications

Amifloxacin (Win49375) showed a consistently high protective effect against bacterial infection, with ED50s of 1.0 mg/kg for p.o. and 1.0 mg/kg for s.c. .

References:

[1]: Cornett J B, Wagner R B, Dobson R A, et al. In vitro and in vivo antibacterial activities of the fluoroquinolone WIN 49375 (amifloxacin)[J]. Antimicrobial agents and chemotherapy, 1985, 27(1): 4-10.

Background

Amifloxacin (Win49375) is a fluorinated quinolone-carboxylic acid antibacterial agent with activity against a broad range of gram-negative organisms. The MICs for 90% of isolates of representative urinary tract pathogens are 0.125µg/ml for Escherichia coli, 0.5µg/ml for Klebsiella pneumoniae, 1.0ug/ml for Proteus vulgaris, 0.5ug/ml for Citrobacter freundii, 2.0µg/ml for Pseudomonas aeruginosa, and 0.5ug/ml for Serratia marcescens [1].

Amifloxacin (Win49375), was an effective drug against experimental infections in mice when given parenterally. Amifloxacin (Win49375) was highly active by the oral route, with 50% effective doses within two- to three fold of those obtained with parenteral medication [2].

References:
[1]. Neu H C, Labthavikul P. Antibacterial activity of amifloxacin (WIN 49, 375), a new quinolone agent[J]. Diagnostic microbiology and infectious disease, 1985, 3(6): 469-478.
[2]. Cornett J B, Wagner R B, Dobson R A, et al. In vitro and in vivo antibacterial activities of the fluoroquinolone WIN 49375 (amifloxacin)[J]. Antimicrobial agents and chemotherapy, 1985, 27(1): 4-10.

Chemical Properties

Cas No. 86393-37-5 SDF
Canonical SMILES O=C(C1=CN(NC)C2=C(C=C(F)C(N3CCN(C)CC3)=C2)C1=O)O
Formula C16H19FN4O3 M.Wt 334.35
Solubility Soluble in DMSO Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 2.9909 mL 14.9544 mL 29.9088 mL
5 mM 0.5982 mL 2.9909 mL 5.9818 mL
10 mM 0.2991 mL 1.4954 mL 2.9909 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 20 reference(s) in Google Scholar.)

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