2-Amino-3-carboxy-1,4-naphthoquinone |
رقم الكتالوجGC64277 |
2-Amino-3-carboxy-1،4-naphthoquinone هو وسيط نقل الإلكترون
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 173043-38-4
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment [1]: | |
Cell lines |
B. longum |
Preparation Method |
Measurement of the native and ACNQ-enhanced NADPH oxidase activity in the cell-free extract of B. longum. The concentrations of NADPH, NADH,and 2-Amino-3-carboxy-1,4-naphthoquinone (ACNQ)were fixed at 1.02 mM, 1.38 mM and 11.2 µM, respectively. |
Reaction Conditions |
Add 1.02 mM NADPH at 200 sec and 11.2 µM ACNQ at 600 sec, respectively. |
Applications |
the ACNQ-enhanced NADH oxidase activity was one third of the ACNQ-enhanced NADPH oxidase activity |
References: [1]: Yamazaki S, Kano K, Ikeda T, et al. Role of 2-amino-3-carboxy-1, 4-naphthoquinone, a strong growth stimulator for bifidobacteria, as an electron transfer mediator for NAD (P)+ regeneration in Bifidobacterium longum[J]. Biochimica et Biophysica Acta (BBA)-General Subjects, 1999, 1428(2-3): 241-250. |
2-amino-3-carboxy-1,4-naphthoquinone (ACNQ) is a growth stimulator for bifidobacteria that is excreted by propionic acid bacteria [1,2,3].
2-amino-3-carboxy-1,4-naphthoquinone is produced and excreted by Propionibacterium freundenreichii, and plays a role as an electron transfer mediator from NAD(P)H to oxygen and hydrogen peroxide in the cytoplasm of the obligate naerobe Bifidobacterium longum. B. longum does not have a respiratory chain and normally regenerates NAD(P)+ by a lactate dehydrogenase-catalyzed reaction, consuming pyruvate and excreting lactate. This process consumes an important intracellular metabolite (e.g., pyruvate), and becomes thermodynamically unfavorable if actate accumulates in the environment. 2-amino-3-carboxy-1,4-naphthoquinone offers an alternative pathway catalyzed by cytosolic diaphorase and peroxidase. By coupling the regeneration of NAD(P)+ to the reduction of O2 and H2O2, 2-amino-3-carboxy-1,4-naphthoquinone stimulates growth and is thought to protect B. longum from oxygen toxicity. The hydrophilicity of 2-amino-3-carboxy-1,4-naphthoquinone increases upon reduction, promoting its accumulation inside the cell. Therefore, only very low concentrations of extracellular ACNQ (i.e., 0.5 nM) are required for it to stimulate the growth of B. longum [4].
References:
[1]. Yamazaki S, Kaneko T, Taketomo N, et al. Glucose metabolism of lactic acid bacteria changed by quinone-mediated extracellular electron transfer[J]. Bioscience, biotechnology, and biochemistry, 2002, 66(10): 2100-2106.
[2]. Yamazaki S, Kano K, Ikeda T, et al. Role of 2-amino-3-carboxy-1, 4-naphthoquinone, a strong growth stimulator for bifidobacteria, as an electron transfer mediator for NAD (P)+ regeneration in Bifidobacterium longum[J]. Biochimica et Biophysica Acta (BBA)-General Subjects, 1999, 1428(2-3): 241-250.
[3]. Mori H, Sato Y, Taketomo N, et al. Isolation and structural identification of bifidogenic growth stimulator produced by Propionibacterium freudenreichii[J]. Journal of Dairy Science, 1997, 80(9): 1959-1964.
[4]. Hernandez M E, Newman D K. Extracellular electron transfer[J]. Cellular and Molecular Life Sciences CMLS, 2001, 58(11): 1562-1571.
Cas No. | 173043-38-4 | SDF | Download SDF |
Formula | C11H7NO4 | M.Wt | 217.18 |
الذوبان | Storage | 4°C, protect from light | |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 4.6045 mL | 23.0224 mL | 46.0448 mL |
5 mM | 0.9209 mL | 4.6045 mL | 9.209 mL |
10 mM | 0.4604 mL | 2.3022 mL | 4.6045 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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