الصفحة الرئيسية>>Nucleotides/Nucleosides>>5-Bromouridine

5-Bromouridine (Synonyms: (–)-5-Bromouridine, BrU, BrUrd, NSC 38296)

رقم الكتالوجGC46681

A brominated uridine analog

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5-Bromouridine التركيب الكيميائي

Cas No.: 957-75-5

الحجم السعر المخزون الكميّة
1g
49٫00
متوفر
5g
98٫00
متوفر

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Sample solution is provided at 25 µL, 10mM.

Description of 5-Bromouridine

5-Bromouridine is a brominated analog of the nucleoside uridine .1 It can be incorporated into RNA and subsequently detected by antibodies against bromodeoxyuridine .2 5-Bromouridine decreases the viability of HL-60 and MOLT-4 cells (LC50s = 10 and 20 μM, respectively). It induces apoptosis and halts the cell cycle at the S phase in HL-60 cells. It is photoreactive, and UV irradiation has been used to cross-link RNA containing 5-bromouridine to proteins in the study of of RNA-protein interactions.1,3 5-Bromouridine can also be incorporated into RNA using 5-bromouridine 5'-triphosphate .

1.Tanner, N.K., Hanna, M.M., and Abelson, J.Binding interactions between yeast tRNA ligase and a precursor transfer ribonucleic acid containing two photoreactive uridine analoguesBiochemistry27(24)8852-8861(1988) 2.Li, X., Patel, R., Melamed, M.R., et al.The cell cycle effects and induction of apoptosis by 5-bromouridine in cultures of human leukaemic MOLT-4 and HL-60 cell lines and mitogen-stimulated normal lymphocytesCell Prolif.27(6)307-319(1994) 3.Gott, J.M., Willis, M.C., Koch, T.H., et al.A specific, UV-induced RNA-protein cross-link using 5-bromouridine-substituted RNABiochemistry30(25)6290-6295(1991)

Chemical Properties of 5-Bromouridine

Cas No. 957-75-5 SDF
المرادفات (–)-5-Bromouridine, BrU, BrUrd, NSC 38296
Canonical SMILES O=C1N([C@]2([H])O[C@H](CO)[C@@H](O)[C@H]2O)C=C(Br)C(N1)=O
Formula C9H11BrN2O6 M.Wt 323.1
الذوبان DMF: 10mg/mL,DMSO: 15mg/mL,PBS (pH 7.2): 5mg/mL Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of 5-Bromouridine

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 3.095 mL 15.4751 mL 30.9502 mL
5 mM 0.619 mL 3.095 mL 6.19 mL
10 mM 0.3095 mL 1.5475 mL 3.095 mL
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In vivo Formulation Calculator (Clear solution) of 5-Bromouridine

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Product Documents

Quality Control & SDS

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Review for 5-Bromouridine

Average Rating: 5 ★★★★★ (Based on Reviews and 40 reference(s) in Google Scholar.)

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