α-Ecdysone |
| رقم الكتالوجGC45204 |
α-إيكديسون هو هرمون ما قبل الهرمون لـ 20-هيدروكسي إيكديسون، وهو هرمون إسترويد يتحكم في تغيرات الحشرات أثناء التسلق.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 3604-87-3
Sample solution is provided at 25 µL, 10mM.
Ecdysone (α-ecdysone) is a steroidal molting hormone that exists in insects and plants and activates mineralocorticoid receptor (MR), leading to glomerular diseases[1]. Ecdysone is also an essential regulator of developmental transitions, including molting and metamorphosis in insects[2].
Ecdysone (0.1, 1, 5, and 10μM for 24h) significantly downregulated the key proteins of the TLR-pathways such as TLR-4, MYD88, IKK-α and IKK-ϒ in RAW 264.7 cells. These proteins were associated with inflammation. Therefore, ecdysone could suppress the TLR pathway to protect cells from inflammation[3].
Ecdysone treatment (6µg/g/d) resulted in evident mesangial deposition of fibronectin and significant reduction in the expression of podocyte-specific molecules such as synaptophysin in male C57BL/6 mice. These phenomena indicated that ecdysone treatment caused glomerulosclerosis and podocyte injury[4].
References:
[1]. Lu M, Wang P, Ge Y, Dworkin L, Brem A, Liu Z, et al. Activation of mineralocorticoid receptor by ecdysone, an adaptogenic and anabolic ecdysteroid, promotes glomerular injury and proteinuria involving overactive GSK3beta pathway signaling. Sci Rep. 2018;8(1):12225. Epub 20180815. doi: 10.1038/s41598-018-29483-7. PubMed PMID: 30111886.
[2]. Xu T, Jiang X, Denton D, Kumar S. Ecdysone controlled cell and tissue deletion. Cell Death Differ. 2020;27(1):1-14. Epub 20191119. doi: 10.1038/s41418-019-0456-9. PubMed PMID: 31745213.
[3].Bhardwaj M, Mamadalieva NZ, Chauhan AK, Kang SC. alpha-Ecdysone suppresses inflammatory responses via the Nrf2 pathway in lipopolysaccharide-stimulated RAW 264.7 cells. Int Immunopharmacol. 2019;73:405-13. Epub 20190529. doi:10.1016/j.intimp.2019.05.038. PubMed PMID: 31152978.
[4]. Lu M, Wang P, Zhou S, Flickinger B, Malhotra D, Ge Y, et al. Ecdysone Elicits Chronic Renal Impairment via Mineralocorticoid-Like Pathogenic Activities. Cell Physiol Biochem. 2018;49(4):1633-45. Epub 20180918. doi: 10.1159/000493499. PubMed PMID: 30227391.
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Cell experiment [1]: |
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Cell lines |
RAW 264.7 cells |
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Preparation method |
α-Ecdysone was dissolved in 5% DMSO and cells were treated with several concentrations of α-ecdysone (0.1, 1, 5 and 10μM) for 24h. |
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Reaction Conditions |
0.1, 1, 5 and 10μM for 24h |
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Applications |
In RAW 264.7 cells , α-ecdysone significantly enhanced lysosomal activity in a concentration dependent fashion. |
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Animal experiment [2]: |
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Animal models |
Male C57BL/6 mice |
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Preparation method |
Male C57BL/6 mice aged 10 weeks received a daily subcutaneous injection of α-ecdysone at 6μg/g/d. After 14 days of treatment, mice were euthanized and kidneys were collected for examinations. |
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Dosage form |
6μg/g/d, subcutaneous injection |
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Applications |
In vivo, daily treatment of mice with α-ecdysone increased cell apoptosis in the kidney, impaired renal function and elicited early signs of renal fibrogenesis. |
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References: [1]. Bhardwaj M, Mamadalieva NZ, Chauhan AK, Kang SC. alpha-Ecdysone suppresses inflammatory responses via the Nrf2 pathway in lipopolysaccharide-stimulated RAW 264.7 cells. Int Immunopharmacol. 2019;73:405-13. Epub 20190529. doi:10.1016/j.intimp.2019.05.038. PubMed PMID: 31152978. [2]. Lu M, Wang P, Zhou S, Flickinger B, Malhotra D, Ge Y, et al. Ecdysone Elicits Chronic Renal Impairment via Mineralocorticoid-Like Pathogenic Activities. Cell Physiol Biochem. 2018;49(4):1633-45. Epub 20180918. doi:10.1159/000493499. PubMed PMID: 30227391. |
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| Cas No. | 3604-87-3 | SDF | |
| Chemical Name | (5β)-2β,3β,14,22R,25-pentahydroxy-cholest-7-en-6-one | ||
| Canonical SMILES | O=C1[C@]2([H])C[C@@H](O)[C@@H](O)C[C@]2(C)[C@]3([H])C([C@@](CC[C@@H]4[C@H](C)[C@H](O)CCC(C)(O)C)(O)[C@]4(C)CC3)=C1 | ||
| Formula | C27H44O6 | M.Wt | 464.6 |
| الذوبان | DMSO:50mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.1524 mL | 10.7619 mL | 21.5239 mL |
| 5 mM | 430.5 μL | 2.1524 mL | 4.3048 mL |
| 10 mM | 215.2 μL | 1.0762 mL | 2.1524 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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