β-Muricholic Acid (Synonyms: 5β-Cholanic Acid-3α,6β,7β-triol, β-MCA) |
| رقم الكتالوجGC40719 |
حمض صفراوي أساسي خاص بالفئران
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Cas No.: 2393-59-1
Sample solution is provided at 25 µL, 10mM.
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β-Muricholic acid is a naturally occurring trihydroxy hydrophilic bile acid, as a biliary cholesterol-desaturating agent and facilitates the dissolution of cholesterol gallstones. It found in mice and differs from human primary bile acids by featuring a hydroxyl group in the β- configuration at the 6-position[1-3].
β-muricholic acid(100 µM；48h) inhibited lipid accumulation in primary-cultured mouse hepatocytes after exposure to palmitic acid (PA)/oleic acid (OA), it may be useful in the treatment of nonalcohol fatty liver disease (NAFLD) [4].
β-muricholic acid(0.5% β-muricholic acid in food; p.o; 8 weeks) is more effective than UDCA in enhancing the dissolution rates of cholesterol gallstones via increasing a liquid crystals-containing phase. Addition of beta-muricholic acid decreased gallstone prevalence to 20% through significantly reducing biliary secretion rate, saturation index, and intestinal absorption of cholesterol that prevented the transition of cholesterol from its liquid crystalline phase to solid crystals and stones [5].
[1]. Denk GU, Kleiss CP, et,al. Tauro-β-muricholic acid restricts bile acid-induced hepatocellular apoptosis by preserving the mitochondrial membrane potential. Biochem Biophys Res Commun. 2012 Aug 10;424(4):758-64. doi: 10.1016/j.bbrc.2012.07.029. Epub 2012 Jul 15. PMID: 22809502.
[2]. Montet JC, Parquet M, et,al. beta-Muricholic acid; potentiometric and cholesterol-dissolving properties. Biochim Biophys Acta. 1987 Mar 13;918(1):1-10. doi: 10.1016/0005-2760(87)90002-6. PMID: 3828364.
[3]. Sacquet E, Parquet M, et,al. Metabolism of beta-muricholic acid in man. Steroids. 1985 May;45(5):411-26. doi: 10.1016/0039-128x(85)90006-6. PMID: 3834660.
[4]. Takada S, Matsubara T, et,al. Stress can attenuate hepatic lipid accumulation via elevation of hepatic β-muricholic acid levels in mice with nonalcoholic steatohepatitis. Lab Invest. 2021 Feb;101(2):193-203. doi: 10.1038/s41374-020-00509-x. Epub 2020 Dec 10. PMID: 33303970.
[5]. Wang DQ, Tazuma S. Effect of beta-muricholic acid on the prevention and dissolution of cholesterol gallstones in C57L/J mice. J Lipid Res. 2002 Nov;43(11):1960-8. doi: 10.1194/jlr.m200297-jlr200. PMID: 12401895.
| Cell experiment [1]: | |
Cell lines | Primary hepatocytes |
Preparation Method | Hepatocytes were exposed to 100 µM β-muricholic acid after treatment with palmitic acid (PA)/oleic acid (OA). |
Reaction Conditions | 100 µM；48h |
Applications | β-muricholic acid inhibited lipid accumulation in primary-cultured mouse hepatocytes after exposure to palmitic acid (PA)/oleic acid (OA). |
| Animal experiment [2]: | |
Animal models | Male C57L/J mice |
Preparation Method | For gallstone dissolution study, mice that have formed cholesterol gallstones were fed chow (control) with or without 0.5% ursodeoxycholic acid (UDCA) or 0.5% β-muricholic acid for 8 weeks. |
Dosage form | 0.5% β-muricholic acid in food; p.o; 8 weeks |
Applications | β-muricholic acid is more effective than UDCA in enhancing the dissolution rates of cholesterol gallstones via increasing a liquid crystals-containing phase. |
References: | |
| Cas No. | 2393-59-1 | SDF | |
| المرادفات | 5β-Cholanic Acid-3α,6β,7β-triol, β-MCA | ||
| Chemical Name | (5β)-3α,6β,7β-trihydroxy-cholan-24-oic acid | ||
| Canonical SMILES | OC(CC[C@@H](C)[C@@]1([H])CC[C@@]2([H])[C@]3([H])[C@@H](O)[C@@H](O)[C@]4([H])C[C@H](O)CC[C@]4(C)[C@@]3([H])CC[C@@]21C)=O | ||
| Formula | C24H40O5 | M.Wt | 408.6 |
| الذوبان | 20mg/ml in ethanol & DMSO, 30mg/ml in DMF | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.4474 mL | 12.2369 mL | 24.4738 mL |
| 5 mM | 0.4895 mL | 2.4474 mL | 4.8948 mL |
| 10 mM | 0.2447 mL | 1.2237 mL | 2.4474 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
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Average Rating: 5 (Based on Reviews and 31 reference(s) in Google Scholar.)
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