Adenosine 5′-diphosphoribose sodium (Synonyms: ADP ribose sodium) |
| رقم الكتالوجGC19729 |
Adenosine 5′-diphosphoribose sodium (ADP ribose sodium) هو مستقلب نيكوتيناميد Adenine nucleotide (NAD +)
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 68414-18-6
Sample solution is provided at 25 µL, 10mM.
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Adenosine 5′-diphosphoribose sodium is a nucleotide derivative and an agonist of Transient receptor potential melastatin 2 (TRPM2), playing an important role in various metabolic pathways within cells[1]. Adenosine 5′-diphosphoribose sodium can modulate intracellular signaling pathways, affecting processes such as cell proliferation, differentiation, and apoptosis[2]. Adenosine 5′-diphosphoribose sodium also plays a key role in cellular stress responses, helping cells cope with adverse conditions like oxidative stress[3]. Adenosine 5′-diphosphoribose sodium is closely related to cellular immune responses, participating in the regulation of immune cell activity[4].
In vitro, treatment of RIN-5F cells with Adenosine 5′-diphosphoribose sodium (100μM) increases intracellular calcium ion (Ca²⁺) concentration through the PLC-IP3 pathway[5]. Treatment of microglial cells with Adenosine 5′-diphosphoribose sodium (1mM) induces Ca²⁺ and Mg²⁺ influx[6].
References:
[1] Fliegert R, Bauche A, Wolf Pérez AM, et al. 2'-Deoxyadenosine 5'-diphosphoribose is an endogenous TRPM2 superagonist. Nat Chem Biol. 2017 Sep;13(9):1036-1044.
[2] Vyas S, Chang P. New PARP targets for cancer therapy. Nat Rev Cancer. 2014 Jul;14(7):502-9.
[3] Dousa TP, Chini EN, Beers KW. Adenine nucleotide diphosphates: emerging second messengers acting via intracellular Ca2+ release. Am J Physiol. 1996 Oct;271(4 Pt 1):C1007-24.
[4] Ji D, Luo ZW, Ovcjak A, et al. Role of TRPM2 in brain tumours and potential as a drug target. Acta Pharmacol Sin. 2022 Apr;43(4):759-770.
[5] Ishii M, Shimizu S, Hagiwara T, et al. Extracellular-added ADP-ribose increases intracellular free Ca2+ concentration through Ca2+ release from stores, but not through TRPM2-mediated Ca2+ entry, in rat beta-cell line RIN-5F. J Pharmacol Sci. 2006 Jun;101(2):174-8.
[6] Kraft R, Grimm C, Grosse K, et al. Hydrogen peroxide and ADP-ribose induce TRPM2-mediated calcium influx and cation currents in microglia. Am J Physiol Cell Physiol. 2004 Jan;286(1):C129-37.
| Cell experiment [1]: | |
Cell lines | RIN-5F cells (rat pancreatic β-cell line) |
Preparation Method | RIN-5F cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum, 100U/ml penicillin, and 100μg/ml streptomycin at 37°C. Cells were treated with extracellular-addedAdenosine 5′-diphosphoribose sodium (100μM) for 10–20 seconds. |
Reaction Conditions | 100μM; 10–20 seconds |
Applications | Extracellular Adenosine 5′-diphosphoribose sodium induced a transient increase in intracellular free Ca²⁺ concentration through Ca²⁺ release from thapsigargin-sensitive Ca²⁺ stores. |
References: | |
| Cas No. | 68414-18-6 | SDF | |
| المرادفات | ADP ribose sodium | ||
| Canonical SMILES | O[C@H]([C@@H]1O)[C@@H](O[C@@H]1COP(OP(OC[C@@H](O)[C@@H](O)[C@@H](O)C=O)([O-])=O)(O)=O)N2C(N=CN=C3N)=C3N=C2.[Na+] | ||
| Formula | C₁₅H₂₂N₅NaO₁₄P₂ | M.Wt | 581.3 |
| الذوبان | Water : 125 mg/mL (215.04 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.7203 mL | 8.6014 mL | 17.2028 mL |
| 5 mM | 344.1 μL | 1.7203 mL | 3.4406 mL |
| 10 mM | 172 μL | 860.1 μL | 1.7203 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
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Quality Control & SDS
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- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 18 reference(s) in Google Scholar.)
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