α-Factor Mating Pheromone, yeast (Mating Factor α) |
| رقم الكتالوجGC30587 |
& alpha؛ -Factor Mating Pheromone، yeast (Mating Factor &alpha؛) هو tridecapeptide الذي يفرزه S.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 59401-28-4
Sample solution is provided at 25 µL, 10mM.
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α-Factor Mating Pheromone, yeast (Mating Factor α) is a 13-amino acid peptide hormone that is continuously synthesized and secreted by MATα-type cells of Saccharomyces cerevisiae and can specifically act on MATa-type cells [1]. α-Factor Mating Pheromone, yeast (Mating Factor α) binds to the G protein-coupled receptor Ste2p on the surface of MATa cells, triggering cell cycle arrest, morphological changes, and the expression of mating-related genes. This factor can compete with radiolabeled ³⁵S-α-factor for binding (Kd = 0.3μM). The binding of α-Factor Mating Pheromone, yeast (Mating Factor α) to the thermosensitive ste2 mutant is thermally unstable, while the binding to other mutants (ste4, ste5, ste7, ste11 and ste12) is not thermally unstable [2]. α-Factor Mating Pheromone, yeast (Mating Factor α) is often used to induce yeast to enter a mating state and is widely used in the secretion and expression of foreign proteins [3].
References:
[1]. Naider F, Becker JM. The α-factor mating pheromone of Saccharomyces cerevisiae: a model for studying the interaction of peptide hormones and G protein-coupled receptors. Peptides. 2004 Sep 1; 25(9): 1441-1463.
[2]. Jenness DD, Burkholder AC, Hartwell LH. Binding of α-factor pheromone to yeast a cells: chemical and genetic evidence for an α-factor receptor. Cell. 1983 Dec 1; 35(2): 521-529.
[3]. Sieber B, Coronas-Serna JM, Martin SG. A focus on yeast mating: From pheromone signaling to cell-cell fusion. InSeminars in Cell & Developmental Biology 2023 Jan 15; 133: 83-95.
| Cas No. | 59401-28-4 | SDF | |
| Canonical SMILES | Trp-His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr | ||
| Formula | C82H114N20O17S | M.Wt | 1684 |
| الذوبان | Soluble in Water | Storage | -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 593.8 μL | 2.9691 mL | 5.9382 mL |
| 5 mM | 118.8 μL | 593.8 μL | 1.1876 mL |
| 10 mM | 59.4 μL | 296.9 μL | 593.8 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 24 reference(s) in Google Scholar.)
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