Biotin-NAD+ |
| رقم الكتالوجGC50505 |
Biotinylated-NAD+; substrate for ADP-ribosylation
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 146385-37-7
Sample solution is provided at 25 µL, 10mM.
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Provides a convenient non-isotopic alternative to radiolabeled NAD+ for determination of IC50 values for candidate PARP inhibitors and studies requiring this substrate. Biotinylated-NAD+ allows an indirect measure of PARP activity when biotin incorporation is detected using a conjugated-streptavidin detection system. Acts as a substrate for ADP-ribosylation. Can be used to label and purify biotinyl-ADP ribosylated proteins. This product is a replacement for R&D Systems product 4670-500-01. 131 μg is supplied as 500 μl of a 0.25 mM solution in water.
Zhang et al (1993) Purification of a nitric oxide-stimulated ADP-ribosylated protein using biotinylated beta-nicotinamide adenine dinucleotide. Biochemistry 32 2228 PMID:8443164 |Yang et al (2017) Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9 Mol. Cell 66 613 PMID:28525742 |Schuster et al (2017) The Hsp90 machinery facilitates the transport of diphtheria toxin into human cells. Sci.Rep. 7 613 PMID:28377614 |Yang (2013) Antitumor activity of a pyrrole-imidazole polyamide. Proc.Natl.Acad.Sci.USA 110 1863 PMID:23319609
| Cas No. | 146385-37-7 | SDF | |
| Canonical SMILES | [H][C@]12CS[C@H]([C@]1(NC(N2)=O)[H])CCCCC(NCCCCCCNC(CNC3=C(N=CN4[C@@H]5O[C@@H]([C@H]([C@H]5O)O)COP(O)(OP(OC[C@H]6O[C@@H]([N+]7=CC=CC(C(N)=O)=C7)[C@H](O)[C@@H]6O)([O-])=O)=O)C4=NC=N3)=O)=O | ||
| Formula | C39H57N11O17P2S | M.Wt | 1045.95 |
| الذوبان | Soluble in water (supplied pre-dissolved at a concentration of 0.25mM) | Storage | Store at -80°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 956.1 μL | 4.7803 mL | 9.5607 mL |
| 5 mM | 191.2 μL | 956.1 μL | 1.9121 mL |
| 10 mM | 95.6 μL | 478 μL | 956.1 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >85.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 33 reference(s) in Google Scholar.)
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