BW-A 78U |
| رقم الكتالوجGC31915 |
BW-A 78U هو مثبط PDE4 مع IC 50 من 3 ميكرومتر
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 101155-02-6
Sample solution is provided at 25 µL, 10mM.
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BW-A 78U is a PDE4 inhibitor with an IC50 of 3 μM.
BW-A 78U is a PDE4 inhibitor with an IC50 of 3 μM. BW-A 78U fails to significantly inhibit arachidonate release. BW-A 78U is ineffective to inhibit the lipopolysaccharide (LPS)-induced TNF-α release[1].
[1]. Boichot E, et al. Anti-inflammatory activities of a new series of selective phosphodiesterase 4 inhibitors derivedfrom 9-benzyladenine. J Pharmacol Exp Ther. 2000 Feb;292(2):647-53.
Cell experiment: | Mononuclear cells are incubated for 30 min with BW-A 78U at the concentration of 10 nM to 10 μM. The cells are then stimulated with lipopolysaccharide (10 μg/mL) overnight at 37°C in an atmosphere of 5% CO2 at 100% humidity. Cell-free supernatants are collected, centrifuged (2000 g), and stored frozen at -20°C before TNF-α determination. TNF-α concentrations in cell culture supernatants are determined by specific ELISA using a commercial kit. Sensitivity of the assay is 1 pg/mL. The absorbance at 450 nm is assessed with an ELISA reader[1]. |
References: [1]. Boichot E, et al. Anti-inflammatory activities of a new series of selective phosphodiesterase 4 inhibitors derivedfrom 9-benzyladenine. J Pharmacol Exp Ther. 2000 Feb;292(2):647-53. | |
| Cas No. | 101155-02-6 | SDF | |
| Canonical SMILES | CNC1=C2N=CN(CC3=CC=CC=C3F)C2=NC=N1 | ||
| Formula | C13H12FN5 | M.Wt | 257.27 |
| الذوبان | DMSO : 150 mg/mL (583.05 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.887 mL | 19.4348 mL | 38.8697 mL |
| 5 mM | 777.4 μL | 3.887 mL | 7.7739 mL |
| 10 mM | 388.7 μL | 1.9435 mL | 3.887 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
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Quality Control & SDS
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- Purity: >99.50%
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