CB-6644 |
| رقم الكتالوجGC34317 |
CB-6644 هو مثبط انتقائي لمركب RUVBL1 / 2 مع نشاط مضاد للسرطانيمنع CB-6644 نشاط ATPase لـ RUVBL1 / 2 مع IC50 من 15 نانومتر
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2316817-88-4
Sample solution is provided at 25 µL, 10mM.
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CB-6644 is a selective inhibitor of RUVBL1/2 complex with anti-cancer activity. CB-6644 blocks the ATPase activity of RUVBL1/2 with an IC50 of 15 nM[1].
CB-6644 (20 µM) interacts with the RUVBL1/2 complex in Ramos cells[1]. CB-6644 (0.4 µM, 80 hours) dramatically inhibits HCT116 cells growth rate and robustly induces apoptosis. Z-VAD-FMK alone does not influence the growth or viability of the cells. However, blockade of caspase activity by Z-VAD-FMK results in a partial recovery of the growth of cells treated with CB-6644[1].|| Cell Viability Assay[1]||Cell Line:|HCT116 cells|Concentration:|0.4 µM|Incubation Time:|80 hours|Result:|Inhibited dramatically cell growth rate.
CB-6644 (150 mg/kg, oral administration, 10 days of treatment for SCID-beige mice bearing Ramos xenograft models, 30 days of treatment for SCID-beige mice bearing RPMI8226 xenograft models) has antitumor activity in xenograft tumor models with tumor growth inhibitions (TGIs) of 68 and 81%, respectively in Ramos xenograft models and RPMI8226 xenograft models[1].|| Animal Model:|SCID-beige mice bearing human tumor xenografts derived from either Burkitt's lymphoma (Ramos) or multiple myeloma (RPMI8226) cell lines[1]|Dosage:|150 mg/kg|Administration:|Oral gavage once (qd) or twice (BID) daily, 10 days of treatment for Ramos, 30 days of treatment for RPMI8226|Result:|There was no significant bodyweight loss in mice. TGI of 68 and 81% in Ramos and RPMI8226, respectively.
[1]. Assimon VA, et al. CB-6644 Is a Selective Inhibitor of the RUVBL1/2 Complex with Anticancer Activity. ACS Chem Biol. 2019 Feb 15;14(2):236-244.
| Cas No. | 2316817-88-4 | SDF | |
| Canonical SMILES | COCC1=C(NC(CC(C)(C)CNC(C2=C(OCC)C=C(F)C(Cl)=C2)=O)=O)C(N(N1CCC3)C4=C3C=CC=C4)=O | ||
| Formula | C29H34ClFN4O5 | M.Wt | 573.06 |
| الذوبان | DMSO: 100 mg/mL (174.50 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.745 mL | 8.7251 mL | 17.4502 mL |
| 5 mM | 0.349 mL | 1.745 mL | 3.49 mL |
| 10 mM | 0.1745 mL | 0.8725 mL | 1.745 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 33 reference(s) in Google Scholar.)
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