Cl-Necrostatin-1 (Synonyms: 7-Cl-Nec-1, 7-Cl-Necrostatin-1, Nec-1f) |
| رقم الكتالوجGC49556 |
A RIPK1 inhibitor
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 862377-51-3
Sample solution is provided at 25 µL, 10mM.
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Cl-Necrostatin-1 is an inhibitor of receptor-interacting protein kinase 1 (RIPK1).1 It inhibits RIPK1 activity when used at concentrations of 0.3, 3, and 30 µM. It also inhibits TNF-α-induced necroptosis in Jurkat cells deficient in Fas-associated death domain protein (FADD; EC50 = 180 nM), a modification that prevents caspase activation in response to death-domain receptor signaling.2,3 Cl-Necrostatin-1 (2 µl of a 4 mM solution, i.c.v.) reduces infarct size when administered pre- and post-occlusion, or post-occlusion only, in a mouse model of middle cerebral artery occlusion (MCAO).
1.Degterev, A., Hitomi, J., Germscheid, M., et al.Identification of RIP1 kinase as a specific cellular target of necrostatinsNat. Chem. Biol.4(5)313-321(2008) 2.Teng, X., Degterev, A., Jagtap, P., et al.Structure-activity relationship study of novel necroptosis inhibitorsBioorg. Med. Chem. Lett.15(22)5039-5044(2005) 3.Degterev, A., Huang, Z., Boyce, M., et al.Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injuryNat. Chem. Biol.1(2)112-119(2005)
| Cas No. | 862377-51-3 | SDF | |
| المرادفات | 7-Cl-Nec-1, 7-Cl-Necrostatin-1, Nec-1f | ||
| Canonical SMILES | O=C(C(N1)CC2=CNC3=C(Cl)C=CC=C32)N(C)C1=S | ||
| Formula | C13H12ClN3OS | M.Wt | 293.8 |
| الذوبان | DMF: 20 mg/ml,DMF:PBS (pH 7.2) (1:1): 0.5 mg/ml,DMSO: 14 mg/ml,Ethanol: 3 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.4037 mL | 17.0184 mL | 34.0368 mL |
| 5 mM | 680.7 μL | 3.4037 mL | 6.8074 mL |
| 10 mM | 340.4 μL | 1.7018 mL | 3.4037 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 13 reference(s) in Google Scholar.)
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