Cy5 NHS ester |
رقم الكتالوجGC12907 |
Cy5 NHS ester (سيانين 5 إن إتش إس استر) هو صبغة تُشعِّ اللون الأحمر لتسمية مجموعات الأحماض الأمينية في الببتيدات والبروتينات والأوليغو نكليوتايد.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Labeling experiment [1]: | |
Preparation Method |
1 mg of Cy5 NHS ester dye was dissolved in 0.1 ml of 62.5 mM sodium tetraborate buffer (pH 8.5). After adding L-methionine in saturation, the mixture was incubated overnight at 4 °C. Fluorophore (Cy5)-conjugated methionine (Cy5-Met) was purified by reverse-phase chromatography on an HPLC system using a C18 column. |
Reaction Conditions |
1 mg Cy5 NHS in 0.1 ml sodium tetraborate buffer |
Applications |
Fluorophore-conjugated methionine was prepared by conjugation of the alpha amino group of L-methionine with Cyanine5 (Cy5) NHS ester dye. |
References: [1]. Kim JM, Seong BL. Highly chromophoric Cy5-methionine for N-terminal fluorescent tagging of proteins in eukaryotic translation systems. Sci Rep. 2017 Sep 14;7(1):11642. |
يعد Cy5 NHS ester (Cyanine5 NHS ester) صبغة تضيء باللون الأحمر لتسمية مجموعات الأحماض الأمينية في الببتيدات والبروتينات والأوليغونكليوتايدات[1]. يتم اثارة هذه المادة عند 646 نانومتر وإطلاقها عند 669 نانومتر[2].
محلول Cy5 NHS ester بتركيز 10 مجم/مل في DMSO. تم استخدام صبغة الإضاءة الحمراء المنبعثة Cy5 NHS ester amine-reactive لتفاعل مع المجموعات الأحادية الأمينية الموجودة على حامض أميني سيستئين التالية لتغطية نهاية خلاصات pBAEs[1]. من ناحية أخرى، تذوب 1 مجم من صبغة Cy5 NHS ester في 0.1 مل من طور بورات الصوديوم (pH 8.5) بتركيز 62.5 ميلِّــــــِـْM . وبعد إضافة L- methionine بالشبع، يُحفظ المزيج على درجة حرارة 4°C طوال الليل. يتم تنقية كاشف (Cy5)-conjugated methionine (Cy5-Met) باستخدام كروماتوغرافی عکسی رابط لهایپیرسیستَەچ Agilent باستخدام ع colon C18[3].
References:
[1].Brugada-Vilà P, Cascante A, et al. Oligopeptide-modified poly(beta-amino ester)s-coated AdNuPARmE1A: Boosting the efficacy of intravenously administered therapeutic adenoviruses. Theranostics. 2020 Feb 3;10(6):2744-2758.
[2].Jiménez Sánchez G, et al. Fluorescent Radiosensitizing Gold Nanoparticles. Int J Mol Sci. 2019 Sep 18;20(18):4618.
[3].Kim JM, Seong BL. Highly chromophoric Cy5-methionine for N-terminal fluorescent tagging of proteins in eukaryotic translation systems. Sci Rep. 2017 Sep 14;7(1):11642.
Cas No. | SDF | ||
Chemical Name | (2E)-1-[6-(2,5-dioxopyrrolidin-1-yl)oxy-6-oxohexyl]-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)penta-2,4-dienylidene]indole-5-sulfonate | ||
Canonical SMILES | CC1(C2=C(C=CC(=C2)S(=O)(=O)[O-])[N+](=C1C=CC=CC=C3C(C4=C(N3CCCCCC(=O)ON5C(=O)CCC5=O)C=CC(=C4)S(=O)(=O)[O-])(C)C)C)C | ||
Formula | C36H40N3NaO10S2 | M.Wt | 761.84 |
الذوبان | very good in water, good in DMF and DMSO | Storage | Store at -20°C, protect from light |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 1.3126 mL | 6.5631 mL | 13.1261 mL |
5 mM | 0.2625 mL | 1.3126 mL | 2.6252 mL |
10 mM | 0.1313 mL | 0.6563 mL | 1.3126 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5
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