الصفحة الرئيسية>>Labeling & Detection>> Cyanine Dyes>>Cy5 NHS ester

Cy5 NHS ester

رقم الكتالوجGC12907

Cy5 NHS ester (سيانين 5 إن إتش إس استر) هو صبغة تُشعِّ اللون الأحمر لتسمية مجموعات الأحماض الأمينية في الببتيدات والبروتينات والأوليغو نكليوتايد.

Products are for research use only. Not for human use. We do not sell to patients.

Cy5 NHS ester التركيب الكيميائي

الحجم السعر المخزون الكميّة
5mg
168٫00
متوفر
25mg
439٫00
متوفر

Tel:(909) 407-4943 Email: sales@glpbio.com

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Sample solution is provided at 25 µL, 10mM.

Product has been cited by 2 publications

Product Documents

Quality Control & SDS

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Protocol

Labeling experiment [1]:

Preparation Method

1 mg of Cy5 NHS ester dye was dissolved in 0.1 ml of 62.5 mM sodium tetraborate buffer (pH 8.5). After adding L-methionine in saturation, the mixture was incubated overnight at 4 °C. Fluorophore (Cy5)-conjugated methionine (Cy5-Met) was purified by reverse-phase chromatography on an HPLC system using a C18 column.

Reaction Conditions

1 mg Cy5 NHS in 0.1 ml sodium tetraborate buffer

Applications

Fluorophore-conjugated methionine was prepared by conjugation of the alpha amino group of L-methionine with Cyanine5 (Cy5) NHS ester dye.

References:

[1]. Kim JM, Seong BL. Highly chromophoric Cy5-methionine for N-terminal fluorescent tagging of proteins in eukaryotic translation systems. Sci Rep. 2017 Sep 14;7(1):11642.

Background

يعد Cy5 NHS ester (Cyanine5 NHS ester) صبغة تضيء باللون الأحمر لتسمية مجموعات الأحماض الأمينية في الببتيدات والبروتينات والأوليغونكليوتايدات[1]. يتم اثارة هذه المادة عند 646 نانومتر وإطلاقها عند 669 نانومتر[2].

محلول Cy5 NHS ester بتركيز 10 مجم/مل في DMSO. تم استخدام صبغة الإضاءة الحمراء المنبعثة Cy5 NHS ester amine-reactive لتفاعل مع المجموعات الأحادية الأمينية الموجودة على حامض أميني سيستئين التالية لتغطية نهاية خلاصات pBAEs[1]. من ناحية أخرى، تذوب 1 مجم من صبغة Cy5 NHS ester في 0.1 مل من طور بورات الصوديوم (pH 8.5) بتركيز 62.5 ميلِّــــــِـْM . وبعد إضافة L- methionine بالشبع، يُحفظ المزيج على درجة حرارة 4°C طوال الليل. يتم تنقية كاشف (Cy5)-conjugated methionine (Cy5-Met) باستخدام كروماتوغرافی عکسی رابط لهایپیرسیستَە‌‌‌‍‍چ Agilent باستخدام ع colon C18[3].

References:
[1].Brugada-Vilà P, Cascante A, et al. Oligopeptide-modified poly(beta-amino ester)s-coated AdNuPARmE1A: Boosting the efficacy of intravenously administered therapeutic adenoviruses. Theranostics. 2020 Feb 3;10(6):2744-2758.
[2].Jiménez Sánchez G, et al. Fluorescent Radiosensitizing Gold Nanoparticles. Int J Mol Sci. 2019 Sep 18;20(18):4618.
[3].Kim JM, Seong BL. Highly chromophoric Cy5-methionine for N-terminal fluorescent tagging of proteins in eukaryotic translation systems. Sci Rep. 2017 Sep 14;7(1):11642.

Chemical Properties

Cas No. SDF
Chemical Name (2E)-1-[6-(2,5-dioxopyrrolidin-1-yl)oxy-6-oxohexyl]-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)penta-2,4-dienylidene]indole-5-sulfonate
Canonical SMILES CC1(C2=C(C=CC(=C2)S(=O)(=O)[O-])[N+](=C1C=CC=CC=C3C(C4=C(N3CCCCCC(=O)ON5C(=O)CCC5=O)C=CC(=C4)S(=O)(=O)[O-])(C)C)C)C
Formula C36H40N3NaO10S2 M.Wt 761.84
الذوبان very good in water, good in DMF and DMSO Storage Store at -20°C, protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 1.3126 mL 6.5631 mL 13.1261 mL
5 mM 0.2625 mL 1.3126 mL 2.6252 mL
10 mM 0.1313 mL 0.6563 mL 1.3126 mL
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