D-(-)-3-Phosphoglyceric Acid (sodium salt) |
| رقم الكتالوجGC43365 |
د - (-) - 3-حمض الفوسفوجليسيريك (3-فوسفو- D- حمض الجليسيريك) ثنائي الصوديوم هو وسيط مهم في عملية التحلل المحفز بالإنزيم.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 80731-10-8
Sample solution is provided at 25 µL, 10mM.
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D-(-)-3-Phosphoglyceric Acid (sodium salt) is an important intermediate in the glycolytic pathway and the Calvin (TCA) cycle, playing a key role in energy metabolism and carbohydrate biosynthesis[1]. In the glycolytic process, D-(-)-3-Phosphoglyceric Acid acts as an intermediate in the conversion of glyceraldehyde-3-phosphate to phosphoenolpyruvate, contributing to the generation of ATP and NADH[2]. In the TCA cycle, D-(-)-3-Phosphoglyceric Acid is formed from the carboxylation of ribulose-1,5-bisphosphate and is subsequently reduced to glyceraldehyde-3-phosphate, which can be used for the synthesis of glucose and other carbohydrates[3]. In plants, D-(-)-3-Phosphoglyceric Acid is also involved in the absorption of carbon dioxide and the synthesis of oxygen in chloroplasts[4].
In vitro, D-(-)-3-Phosphoglyceric Acid increases the activity of bisphosphoglycerate synthase in red blood cells in a dose-dependent manner[5]. After HEK293 cells were cultured in low glucose conditions (<5mM) for 2 hours, the addition of D-(-)-3-Phosphoglyceric Acid (10, 25, 50, 100, 150, 300μM) inhibited the interaction between phosphoglycerate dehydrogenase (PHGDH) and p53, triggered the phosphorylation of p53 at Ser46, and thereby induced apoptosis and inhibited cell proliferation[6].
References:
[1] VON KORFF RW. Interaction of glycolytic and mitochondrial enzyme systems. II. The reaction sequences: fructose diphosphate to 3-phosphoglyceric acid; and pyruvate to lactate, carbon dioxide and water. Biochim Biophys Acta. 1959 Feb;31(2):467-75.
[2] Lee K, Doello S, Hagemann M, Forchhammer K. Deciphering the tight metabolite-level regulation of glucose-1-phosphate adenylyltransferase (GlgC) for glycogen synthesis in cyanobacteria. FEBS J. 2025 Feb;292(4):759-775.
[3] Ramos A, Neves AR, Ventura R, et al. Effect of pyruvate kinase overproduction on glucose metabolism of Lactococcus lactis. Microbiology (Reading). 2004 Apr;150(Pt 4):1103-1111.
[4] Routier C, Vallan L, Daguerre Y, et al. Chitosan-Modified Polyethyleneimine Nanoparticles for Enhancing the Carboxylation Reaction and Plants' CO2 Uptake. ACS Nano. 2023 Feb 28;17(4):3430-3441.
[5] Yu KT, Pendley C 2nd, Herczeg T, et al. 2,3-Diphosphoglycerate phosphatase/synthase: a potential target for elevating the diphosphoglycerate level in human red blood cells. J Pharmacol Exp Ther. 1990 Jan;252(1):192-200.
[6] Wu YQ, Zhang CS, Xiong J, et al. Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine synthesis to p53 activation to control cell fate. Cell Res. 2023 Nov;33(11):835-850.
| Cell experiment [1]: | |
Cell lines | HEK293 cells |
Preparation Method | HEK293 cells were incubated for 2 hours under low glucose conditions (<5mM) and then added with D-(-)-3-Phosphoglyceric Acid (sodium salt) (10, 25, 50, 100, 150, 300μM) and incubated for two hours. |
Reaction Conditions | 0, 10, 25, 50, 100, 150, and 300μM; 2h. |
Applications | D-(-)-3-Phosphoglyceric acid (sodium salt) can inhibit the interaction between phosphoglycerate dehydrogenase (PHGDH) and p53, trigger phosphorylation at Ser46 of p53, thereby inducing apoptosis and inhibiting the proliferation of HEK293 cells. |
References: | |
| Cas No. | 80731-10-8 | SDF | |
| Canonical SMILES | [O-]C([C@H](O)COP(O)([O-])=O)=O.[Na+].[Na+] | ||
| Formula | C3H5O7P•2Na | M.Wt | 230 |
| الذوبان | PBS (pH 7.2): 10 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 4.3478 mL | 21.7391 mL | 43.4783 mL |
| 5 mM | 869.6 μL | 4.3478 mL | 8.6957 mL |
| 10 mM | 434.8 μL | 2.1739 mL | 4.3478 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >93.00%
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- SDS (Safety Data Sheet)
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Average Rating: 5 (Based on Reviews and 14 reference(s) in Google Scholar.)
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