Dihydrorhodamine 123 (DHR 123) (Synonyms: DHR 123) |
رقم الكتالوجGC30581 |
Dihydrorhodamine 123 (DHR 123) (DHR 123) هو مسبار فلوري (&Lambda ؛ ex = 488 nm ، &Lambda ؛ em = 525 nm).
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Cas No.: 109244-58-8
Sample solution is provided at 25 µL, 10mM.
Dihydrorhodamine 123 (DHR 123) is a non-fluorescent precursor that transforms into the fluorescent compound rhodamine 123 (Rh123+) upon oxidation. This transformation process makes DHR 123 a useful tool for detecting reactive oxygen species (ROS) within cells. Dihydrorhodamine 123 can cross the cell membrane, and once inside the cell, it is oxidized by ROS into Rh123+, producing green fluorescence that accumulates in the mitochondrial matrix. The excitation wavelength of Rh123+ is 485 nm, and its emission wavelength is 535 nm[1]. Dihydrorhodamine 123 can also be used as an indicator for the formation of intracellular peroxynitrite[2].
References:
[1] Wing-Kee Lee, Stephanie Probst, Bettina Scharner, et al. Distinct concentration-dependent oxidative stress profiles by cadmium in a rat kidney proximal tubule cell line[J] Archives of Toxicology. 30 January 2024.
[2] Crow, J.P. Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: Implications for intracellular measurement of reactive nitrogen and oxygen species Nitric Oxide1(2)145-157(1997).
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