الصفحة الرئيسية>>Natural Products>>Diphenylterazine (DTZ)

Diphenylterazine (DTZ) (Synonyms: DTZ)

رقم الكتالوجGC33428

ديفينيلتيرازين (DTZ) هو عامل الإضاءة الحيوية.

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Diphenylterazine (DTZ) التركيب الكيميائي

Cas No.: 344940-63-2

الحجم السعر المخزون الكميّة
1mg
105٫00
متوفر
5mg
210٫00
متوفر
10mg
329٫00
متوفر
50mg
686٫00
متوفر

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مراجعات العميل

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

View current batch:

Protocol

Cell experiment [1]:

Cell lines

HEK 293T

Preparation Method

Cell viability was determined using RealTime-Glo MT Cell Viability Assay (Promega) after incubation of HEK 293T cells with individual luciferin substrates for 24 h at 37 °C. Cell morphology was further evaluated using microscopy.

Reaction Conditions

24h 30 μM Diphenylterazine

Applications

Compared to other substrates,As a novel luciferase substrate based on CTZ analogue, Diphenylterazine has bright red-shift bioluminescence. Moreover, Diphenylterazine alone yielded very little background and thus exhibited excellent signal-to-background ratios , Furthermore, Diphenylterazine elicited minimal cell toxicity at millimolar concentrations.

Animal experiment [2]:

Animal models

BALB/c mice

Preparation Method

Bioluminescence imaging of intravenously injected HEK 293T cells:BALB/c mice are used and transfected with cells expressing biotinase gene by injecting cells into the tail vein of BALB/c mice. After the diminishing of the bioluminescence, 0.3 µmol Diphenylterazine is intraperitoneally injected. Mice are imaged with a 1-min exposure per frame over a course of 20 min.

Dosage form

0.3 µmol Diphenylterazine

Applications

Diphenylterazine was used as an emerging luciferase reporter substrate in mice. The imaging of deep tissue targets in mice was evaluated by injecting luciferase gene expressing HEK 293T cells through the tail vein. First, Diphenylterazine as a substrate has no background in control mice, and when injected into mice transfected with the luciferase gene, the fluorescence emitted by Diphenylterazine can be detected, and the intensity of Diphenylterazine has increased significantly compared with other substrates.

References:

[1]. Yeh HW, Karmach O, Ji A, Carter D, Martins-Green MM, Ai HW. Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging. Nat Methods. 2017 Oct;14(10):971-974. doi: 10.1038/nmeth.4400. Epub 2017 Sep 4. PMID: 28869756; PMCID: PMC5678970.

Background

الديفينيلتيرازين، وهو مشتق من CTZ، هو عامل جيد للوسائط الحافزة لإضاءة البلازما. يعد الديفينيلتيرازين عاملاً حيويًا. يؤدي استخدام الديفينيلتيرازين بمفرده إلى تولِّد خلفية ضئيلة جدًا، مما يؤدي إلى نسبة إشارة-خلفية ممتازة. وعلاوة على ذلك، فإن استخدام الديفينيلتيرازين له أثر قليل في سُمِّية الخلايا بالجرعات المولارية الملحوظة. كذلك، يُمكَّن تصنِّع الديفْینیْل‌- التیراسین من المستحضرات التجارية غير المكلَّفَۃ في خطوتَین باستحقابات ممتازۃ[1].

في التجارب الخلوية، يمكننا رؤية أن ديفينيلتيرازين لديها نسبة إشارة إلى ضوضاء أقل من المركبات الأخرى، وديفينيلتيرازين يسبب سمية خلايا حد أدنى عند تركيزات ميللمولار.

على العكس من ذلك، تسببت أنواع أخرى من المواد الأساسية المختبرة في وفاة الخلايا داخل نطاق تركيز المادة الأساسية المختبرة.

في تجربة الفأر، كانت سطوع الإضاءة الحيوية لموقع السطح، عند تركيز المادة الخاملة 0.1 ملمولار، أعلى من ذلك في المجموعة التحكمية.

كان الأمر نفسه صحيحًا لتصوير أهداف الأنسجة العميقة، ولم تنتج حقن Diphenylterazine في فئران BALB/c غير المحولة أي انبعاث خلفية، وكان بإمكان التشعيع ما زال قابلاً للكشف عند حقنه داخل الوريد في الفئران[2].

References:
[1].Tian X, Zhang Y, Li X, Xiong Y, Wu T, Ai HW. A luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice. Nat Commun. 2022 Jul 8;13(1):3967. doi: 10.1038/s41467-022-31673-x. PMID: 35803917; PMCID: PMC9270435.
[2]. Yeh HW, et al. Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging. Nat Methods. 2017 Oct;14(10):971-974.

Chemical Properties

Cas No. 344940-63-2 SDF
المرادفات DTZ
Canonical SMILES O=C1C(CC2=CC=CC=C2)=NC3=C(C4=CC=CC=C4)NC(C5=CC=CC=C5)=CN31
Formula C25H19N3O M.Wt 377.44
الذوبان DMF : 11 mg/mL Storage Store at -20°C, protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 2.6494 mL 13.2471 mL 26.4943 mL
5 mM 0.5299 mL 2.6494 mL 5.2989 mL
10 mM 0.2649 mL 1.3247 mL 2.6494 mL
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Average Rating: 5 ★★★★★ (Based on Reviews and 4 reference(s) in Google Scholar.)

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