Evans Blue tetrasodium salt |
| رقم الكتالوجGC13947 |
يعتبر ملح إيفانز الأزرق رباعي الصوديوم مثبطًا قويًا لامتصاص L-glutamate عبر ناقل الأحماض الأمينية المثير للغشاء (EAAT).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 314-13-6
Sample solution is provided at 25 µL, 10mM.
Evans Blue tetrasodium salt is a highly potent inhibitor of the vesicular glutamate uptake (IC50=87nM)[1]. Evans Blue is widely used in biomedicine including the estimation of blood volume, the assessment of vascular permeability to macromolecules, the detection of lymph nodes, and the location of the tumor lesions[2].
Evans Blue (0-100μM, 1h) potently reduced Epsilon-toxin (ETX)-induced hemolysis (EC50: ~1.2μM)[3].
Evans Blue (2%, 4ml/kg; i.v.; 50min) combined with FITC-Dextran (2000kDa) can be used to quantify blood-brain barrier (BBB) leakage in specific regions of mice with lipopolysaccharide-induced BBB disruption and in apoE-/- mice[4].
References:
[1] Roseth S, Fykse EM, Fonnum F. Uptake of l‐glutamate into rat brain synaptic vesicles: effect of inhibitors that bind specifically to the glutamate transporter. Journal of neurochemistry. 1995 Jul;65(1):96-103.
[2] Yao L, Xue X, Yu P, et al. Evans blue dye: a revisit of its applications in biomedicine. Contrast media & molecular imaging. 2018;2018(1):7628037.
[3] Gao J, Xin W, Huang J, et al. Hemolysis in human erythrocytes by Clostridium perfringens epsilon toxin requires activation of P2 receptors. Virulence. 2018 Dec 31;9(1):1601-14.
[4] Xu Y, He Q, Wang M, et al. Quantifying blood-brain-barrier leakage using a combination of evans blue and high molecular weight FITC-Dextran. Journal of neuroscience methods. 2019 Sep 1;325:108349.
| Cell experiment [1]: | |
Cell lines | Erythrocytes isolated from healthy volunteers |
Preparation Method | Human blood was collected by venopuncture from healthy volunteers using evacuated blood collection tubes containing EDTA-2K. The blood of mice, rats, sheep, bovine, rabbits, monkeys, horses, dogs and goats were collected, then the erythrocytes were washed three times in 0.01M PBS (1,000g, 4°C, 5min). The “buffy coat” was removed and isolated erythrocytes were packed by centrifugation at 1,000g for 10min at 4°C. Washed erythrocytes were resuspended in 0.01M PBS to produce a 5% erythrocyte solution. The hemolytic activity was measured spectrophotometrically at 540nm. In the hemolytic assay, purified recombinant ETX (rETX) (0 ~ 33μM) and increasing concentrations of Evans Blue was added to the 5% red blood cell solution (final concentration of 3.3%) and incubated up to 1h at 37°C with constant swirling (300rpm). Then the 1.5ml tubes were centrifuged at 1,000g for 10min at 4°C, and the optical density at 540nm of the supernatants was determined as a measure of the released hemoglobin. |
Reaction Conditions | 0-100μM, 1h |
Applications | Evans Blue potently reduced Epsilon-toxin (ETX)-induced hemolysis (EC50: ~1.2μM). |
| Animal experiment [2]: | |
Animal models | Blood-brain barrier mouse model |
Preparation Method | Eight C57BL/6J mice were intraperitoneally injected with 1mg/ml LPS (4ml/kg, diluted in normal saline) to induce BBB disruption. Another eight C57BL/6J mice were intraperitoneally injected with normal saline (4ml/kg) as control (LPS-control). Six hours after the injection, mice were anesthetized with 1.5% pentobarbital sodium (3ml/kg, i.p.), then cannulated with a 26 Ga catheter through the tail vein. 2% Evans Blue (4ml/kg, diluted in normal saline) was injected through the catheter. 50min after EB circulation, 6mg/ml FITC-Dextran (4ml/kg, diluted in Normal Saline) was injected through the catheter and circulated for 10min. |
Dosage form | 2%, 4ml/kg; i.v.; 50min |
Applications | Evans Blue combined with FITC-Dextran (2000kDa) can be used to quantify blood-brain barrier (BBB) leakage in specific regions of mice with lipopolysaccharide-induced BBB disruption and in apoE-/- mice. |
References: | |
| Cas No. | 314-13-6 | SDF | |
| Chemical Name | sodium (6Z,6'Z)-6,6'-(2,2'-(3,3'-dimethyl-[1,1'-biphenyl]-4,4'-diyl)bis(hydrazin-2-yl-1-ylidene))bis(4-amino-5-oxo-5,6-dihydronaphthalene-1,3-disulfonate) | ||
| Canonical SMILES | O=C1C(C(C=C/C1=N/NC(C(C)=C2)=CC=C2C3=CC=C(C(C)=C3)N/N=C4C(C(C(C=C\4)=C5S([O-])(=O)=O)=C(C(S([O-])(=O)=O)=C5)N)=O)=C6S([O-])(=O)=O)=C(C(S([O-])(=O)=O)=C6)N.[Na+].[Na+].[Na+].[Na+] | ||
| Formula | C34H24N6Na4O14S4 | M.Wt | 960.82 |
| الذوبان | ≥ 192.4 mg/mL in DMSO, ≥ 213.8 mg/mL in Water | Storage | Store at RT |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.0408 mL | 5.2039 mL | 10.4078 mL |
| 5 mM | 0.2082 mL | 1.0408 mL | 2.0816 mL |
| 10 mM | 0.1041 mL | 0.5204 mL | 1.0408 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 22 reference(s) in Google Scholar.)
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