الصفحة الرئيسية>>Signaling Pathways>> Proteases>> Endogenous Metabolite>>Galactose 1-phosphate

Galactose 1-phosphate

رقم الكتالوجGC30337

جلاكتوز 1-فوسفات هو وسيط في عملية استقلاب الجلاكتوز والسكريات النوكليوتيدية.

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Galactose 1-phosphate التركيب الكيميائي

Cas No.: 2255-14-3

الحجم السعر المخزون الكميّة
10mg
46٫00
متوفر
50mg
110٫00
متوفر

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Cell experiment [1]:

Cell lines

Fibroblasts derived from the foreskin of 3-8-day-old healthy neonates

Preparation Method

Galactose 1-phosphate (Gal-1-P) at different concentrations (1-16 mM) was preincubated separately with whole brain homogenates at 37℃ for 1 h.

Reaction Conditions

0-10 mM;1-14 days

Applications

Gal-1-P can stimulated rat brain Na+,K+-ATPase.

Animal experiment [2]:

Animal models

Preparation Method

Cells were cultured for 1-14 days with 0-10 mM Gal-1-P and then stimulated with 5% fetal bovine serum (FBS) or 50 ng/mL of platelet-derived growth factor (PDGF) or fibroblast growth factor (FGF) or IGF-1 for 24 h.

Dosage form

Applications

Gal-1-P (5-10 mM) in culture medium for 7-14 days significantly decreased IGF-1-, PDGF-and FBS-stimulated DNA synthesis.

References:

[1]. Tsakiris S, Marinou K,et,al. The effect of galactose metabolic disorders on rat brain Na+,K+-ATPase activity. Z Naturforsch C J Biosci. 2002 Sep-Oct;57(9-10):939-43. doi: 10.1515/znc-2002-9-1030. PMID: 12440737.
[2]. Al-Essa M, Dhaunsi G. Receptor-mediated attenuation of insulin-like growth factor-1 activity by galactose-1-phosphate in neonate skin fibroblast cultures: Galactosemia pathogenesis. Adv Clin Exp Med. 2020 Apr;29(4):499-504. doi: 10.17219/acem/111807. PMID: 32356415.

Background

جلاكتوز 1-فوسفات (Gal-1-P) هي مادة الأساس لإنزيم جالاكتوز 1-فوسفات يوريديليل ترانسفيراز (GALT)، وهي سامة ولها تأثير مثبط على العديد من الإنزيمات في مسار استقلاب الجلوكوز [1،2]. يمكن أن يحفِّز Gal-1-P (1-16 مم؛ 37 ℃ ؛ 1 ساعة) ناقل صوديوم بروتية كبيرة -ATPase في دماغ الجرذان [3].

Gal-1-P (5-10 ملم) في وسط الثقافة لمدة 7-14 يومًا خفض بشكل كبير تخليق الحمض النووي المحفز بواسطة IGF-1 و PDGF و FBS في خلايا الألياف[4]. تمت معالجة التراكيز المختلفة (1-16 ميلي مولار) من فوسفات جلاكتوز -1 (Gal-1-P) قبل التجانس لأدمغة رضع الجرذان وكذلك AChE نقية من سمك الإيل Electroforus electricus عند 37 درجة مئوية لمدة ساعة. خفض Gal-1-P نشاط الإنزيم تدريجيًا حتى وصوله إلى هضبة عند التجانس بالتراكيز 8 -16 ميلِّ. يستطيع Gal-1-P أن يؤثر على تحلل أستيل كولين باعتباره يعامَِلاً بشكََََََُُُُِِِْروٍ على جزء AChE [5].

References:
[1]. Oh SL, Cheng LY, et,al. Galactose 1-phosphate accumulates to high levels in galactose-treated cells due to low GALT activity and absence of product inhibition of GALK. J Inherit Metab Dis. 2020 May;43(3):529-539. doi: 10.1002/jimd.12198. Epub 2019 Dec 17. PMID: 31774565.
[2]. Lai K, Langley SD, et,al. GALT deficiency causes UDP-hexose deficit in human galactosemic cells. Glycobiology. 2003 Apr;13(4):285-94. doi: 10.1093/glycob/cwg033. Epub 2003 Jan 3. PMID: 12626383.
[3]. Tsakiris S, Marinou K, et,al. The effect of galactose metabolic disorders on rat brain Na+,K+-ATPase activity. Z Naturforsch C J Biosci. 2002 Sep-Oct;57(9-10):939-43. doi: 10.1515/znc-2002-9-1030. PMID: 12440737.
[4]. Al-Essa M, Dhaunsi G. Receptor-mediated attenuation of insulin-like growth factor-1 activity by galactose-1-phosphate in neonate skin fibroblast cultures: Galactosemia pathogenesis. Adv Clin Exp Med. 2020 Apr;29(4):499-504. doi: 10.17219/acem/111807. PMID: 32356415.
[5]. Tsakiris S, Schulpis KH. The effect of galactose metabolic disorders on rat brain acetylcholinesterase activity. Z Naturforsch C J Biosci. 2000 Sep-Oct;55(9-10):852-5. doi: 10.1515/znc-2000-9-1032. PMID: 11098845.

Chemical Properties

Cas No. 2255-14-3 SDF
Canonical SMILES O=P(O)(O)O[C@H]1O[C@@H]([C@H](O)[C@H](O)[C@H]1O)CO
Formula C6H13O9P M.Wt 260.14
الذوبان Water:125 mg/mL (371.67 mM) Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 3.8441 mL 19.2204 mL 38.4408 mL
5 mM 0.7688 mL 3.8441 mL 7.6882 mL
10 mM 0.3844 mL 1.922 mL 3.8441 mL
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