Galactose 1-phosphate |
رقم الكتالوجGC30337 |
جلاكتوز 1-فوسفات هو وسيط في عملية استقلاب الجلاكتوز والسكريات النوكليوتيدية.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2255-14-3
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment [1]: | |
Cell lines |
Fibroblasts derived from the foreskin of 3-8-day-old healthy neonates |
Preparation Method |
Galactose 1-phosphate (Gal-1-P) at different concentrations (1-16 mM) was preincubated separately with whole brain homogenates at 37℃ for 1 h. |
Reaction Conditions |
0-10 mM;1-14 days |
Applications |
Gal-1-P can stimulated rat brain Na+,K+-ATPase. |
Animal experiment [2]: | |
Animal models |
|
Preparation Method |
Cells were cultured for 1-14 days with 0-10 mM Gal-1-P and then stimulated with 5% fetal bovine serum (FBS) or 50 ng/mL of platelet-derived growth factor (PDGF) or fibroblast growth factor (FGF) or IGF-1 for 24 h. |
Dosage form |
|
Applications |
Gal-1-P (5-10 mM) in culture medium for 7-14 days significantly decreased IGF-1-, PDGF-and FBS-stimulated DNA synthesis. |
References: [1]. Tsakiris S, Marinou K,et,al. The effect of galactose metabolic disorders on rat brain Na+,K+-ATPase activity. Z Naturforsch C J Biosci. 2002 Sep-Oct;57(9-10):939-43. doi: 10.1515/znc-2002-9-1030. PMID: 12440737. |
جلاكتوز 1-فوسفات (Gal-1-P) هي مادة الأساس لإنزيم جالاكتوز 1-فوسفات يوريديليل ترانسفيراز (GALT)، وهي سامة ولها تأثير مثبط على العديد من الإنزيمات في مسار استقلاب الجلوكوز [1،2]. يمكن أن يحفِّز Gal-1-P (1-16 مم؛ 37 ℃ ؛ 1 ساعة) ناقل صوديوم بروتية كبيرة -ATPase في دماغ الجرذان [3].
Gal-1-P (5-10 ملم) في وسط الثقافة لمدة 7-14 يومًا خفض بشكل كبير تخليق الحمض النووي المحفز بواسطة IGF-1 و PDGF و FBS في خلايا الألياف[4]. تمت معالجة التراكيز المختلفة (1-16 ميلي مولار) من فوسفات جلاكتوز -1 (Gal-1-P) قبل التجانس لأدمغة رضع الجرذان وكذلك AChE نقية من سمك الإيل Electroforus electricus عند 37 درجة مئوية لمدة ساعة. خفض Gal-1-P نشاط الإنزيم تدريجيًا حتى وصوله إلى هضبة عند التجانس بالتراكيز 8 -16 ميلِّ. يستطيع Gal-1-P أن يؤثر على تحلل أستيل كولين باعتباره يعامَِلاً بشكََََََُُُُِِِْروٍ على جزء AChE [5].
References:
[1]. Oh SL, Cheng LY, et,al. Galactose 1-phosphate accumulates to high levels in galactose-treated cells due to low GALT activity and absence of product inhibition of GALK. J Inherit Metab Dis. 2020 May;43(3):529-539. doi: 10.1002/jimd.12198. Epub 2019 Dec 17. PMID: 31774565.
[2]. Lai K, Langley SD, et,al. GALT deficiency causes UDP-hexose deficit in human galactosemic cells. Glycobiology. 2003 Apr;13(4):285-94. doi: 10.1093/glycob/cwg033. Epub 2003 Jan 3. PMID: 12626383.
[3]. Tsakiris S, Marinou K, et,al. The effect of galactose metabolic disorders on rat brain Na+,K+-ATPase activity. Z Naturforsch C J Biosci. 2002 Sep-Oct;57(9-10):939-43. doi: 10.1515/znc-2002-9-1030. PMID: 12440737.
[4]. Al-Essa M, Dhaunsi G. Receptor-mediated attenuation of insulin-like growth factor-1 activity by galactose-1-phosphate in neonate skin fibroblast cultures: Galactosemia pathogenesis. Adv Clin Exp Med. 2020 Apr;29(4):499-504. doi: 10.17219/acem/111807. PMID: 32356415.
[5]. Tsakiris S, Schulpis KH. The effect of galactose metabolic disorders on rat brain acetylcholinesterase activity. Z Naturforsch C J Biosci. 2000 Sep-Oct;55(9-10):852-5. doi: 10.1515/znc-2000-9-1032. PMID: 11098845.
Cas No. | 2255-14-3 | SDF | |
Canonical SMILES | O=P(O)(O)O[C@H]1O[C@@H]([C@H](O)[C@H](O)[C@H]1O)CO | ||
Formula | C6H13O9P | M.Wt | 260.14 |
الذوبان | Water:125 mg/mL (371.67 mM) | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 3.8441 mL | 19.2204 mL | 38.4408 mL |
5 mM | 0.7688 mL | 3.8441 mL | 7.6882 mL |
10 mM | 0.3844 mL | 1.922 mL | 3.8441 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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