الصفحة الرئيسية>>Signaling Pathways>> TGF-β / Smad Signaling>> PKC>>Desmethylglycitein

Desmethylglycitein (Synonyms: 6-hydroxy Daidzein, 6,7,4’-THIF)

رقم الكتالوجGC38482

Desmethylglycitein (4 '، 6،7-Trihydroxyisoflavone) ، مستقلب من ديدزين ، مصدره Glycine max مع مضادات الأكسدة وأنشطة مكافحة السرطان. يرتبط Desmethylglycitein مباشرة بـ CDK1 و CDK2 في الجسم الحي ، مما يؤدي إلى قمع نشاط CDK1 و CDK2Desmethylglycitein هو مثبط مباشر لبروتين كيناز C (PKC) α ، ضد الأشعة فوق البنفسجية الشمسية (sUV) التي يسببها مصفوفة المصفوفة metalloproteinase 1 (MMP1)يرتبط Desmethylglycitein بـ PI3K بطريقة تنافسية ATP في العصارة الخلوية ، حيث يثبط نشاط PI3K وشلالات الإشارات النهائية ، مما يؤدي إلى قمع تكون الدهون في الخلايا الأولية 3T3-L1

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Desmethylglycitein التركيب الكيميائي

Cas No.: 17817-31-1

الحجم السعر المخزون الكميّة
1mg
234٫00
متوفر
5mg
699٫00
متوفر

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Sample solution is provided at 25 µL, 10mM.

Description of Desmethylglycitein

Desmethylglycitein (4',6,7-Trihydroxyisoflavone), a metabolite of daidzein, sourced from Glycine max with antioxidant, and anti-cancer activities.Desmethylglycitein binds directly to CDK1 and CDK2 in vivo, resulting in the suppresses CDK1 and CDK2 activity[1]. Desmethylglycitein is a direct inhibitor of protein kinase C (PKC)α, against solar UV (sUV)-induced matrix matrix metalloproteinase 1 (MMP1)[2]. Desmethylglycitein binds to PI3K in an ATP competitive manner in the cytosol, where it inhibits the activity of PI3K and downstream signaling cascades, leading to the suppression of adipogenesis in 3T3-L1 preadipocytes[3].

Desmethylglycitein (4',6,7-Trihydroxyisoflavone)(0-100 μM; 24-72 hours) suppesses anchorage-dependent growth of HCT-116 and DLD1 cells in a dose- and time-dependent manner without cytotoxicity [1].Desmethylglycitein (4',6,7-Trihydroxyisoflavone)(0-100 μM; 24-72 hours) suppresses CDK1 activity in a dose-dependent manner and inhibits CDK2 activity in HCT-116 cells[1].Desmethylglycitein (4',6,7-Trihydroxyisoflavone)(0-100 μM; 24-72 hours) induces cell cycle arrest at the S and G2/M phases, the percentage of cells in S phase is higher in the 100 μM 6,7,4′-THIF-treated group, and the same pattern is observed in G2/M phase (29.5% versus 19.1% ) [1]. Cell Viability Assay[1] Cell Line: HCT-116 cells

Desmethylglycitein (4',6,7-Trihydroxyisoflavone) (intraperitoneally injection; 5 or 25 mg/kg; once daily; 20 days) suppresses tumor development in mice and serves as an effective anticancer treatment with the potential to inhibit or delay the tumorigenicity of HCT-116 cells in an in vivo system[1]. Animal Model: Female athymic nude mice subcutaneously injected with HCT-116 cells[1]

[1]. Lee DE, et al. 6,7,4'-trihydroxyisoflavone inhibits HCT-116 human colon cancer cell proliferation by targeting CDK1 and CDK2. Carcinogenesis. 2011 Apr;32(4):629-35. [2]. Lim TG, et al. The daidzein metabolite, 6,7,4'-Trihydroxyisoflavone, is a novel inhibitor of PKCα in suppressing solar UV-induced matrix metalloproteinase 1. Int J Mol Sci. 2014 Nov 19;15(11):21419-32 [3]. Seo SG, et al. A metabolite of daidzein, 6,7,4'-trihydroxyisoflavone, suppresses adipogenesis in 3T3-L1 preadipocytes via ATP-competitive inhibition of PI3K.

Chemical Properties of Desmethylglycitein

Cas No. 17817-31-1 SDF
المرادفات 6-hydroxy Daidzein, 6,7,4’-THIF
Canonical SMILES O=C1C(C2=CC=C(O)C=C2)=COC3=CC(O)=C(O)C=C13
Formula C15H10O5 M.Wt 270.24
الذوبان DMSO: 125 mg/mL (462.55 mM) Storage Store at 2-8°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of Desmethylglycitein

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1 mg 5 mg 10 mg
1 mM 3.7004 mL 18.5021 mL 37.0041 mL
5 mM 0.7401 mL 3.7004 mL 7.4008 mL
10 mM 0.37 mL 1.8502 mL 3.7004 mL
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