Gly-Pro-pNA (hydrochloride) (Synonyms: Gly-Pro pnitroanilide, GP-pNA) |
| رقم الكتالوجGC18201 |
Gly-Pro-pNA (hydrochloride) is a chromogenic substrate that can be cleaved by the circulating enzyme, dipeptidyl peptidase IV (DPP IV).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 103213-34-9
Sample solution is provided at 25 µL, 10mM.
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Gly-Pro-pNA (hydrochloride) is a chromogenic substrate that can be cleaved by the circulating enzyme, dipeptidyl peptidase IV (DPP IV). Enzyme activity can be quantified by colorimetric detection of free p-nitroanilide at 405 nm. This Gly-Pro-pNA substrate can be used to screen for DPP IV inhibitors.[5].
Mare whey proteins show good DPP-IV inhibitory activity in vitro and can inhibit DPP-IV from degrading Gly-Pro-pNA (hydrochloride) Gly-Pro-pNA (hydrochloride) [1]. The in vitro DPP-IV inhibitory activities of quinoa protein hydrolysates can be determined using Gly-Pro-pNA (hydrochloride) as a substrate[2]. Gly-Pro-pNA (hydrochloride) can also be used to evaluate the activity of DPP-IV in human serum[3]. Demonstrating that DPP IV in diabetic patients is not very different from that in normal conditions[4]. Gly-pro p-nitroanilide hydrochloride was used as a substrate to detect DPPIV levels in patients with Polycystic syndrome (PCOS), a deregulation of DPP4 serum levels could be an additional characteristic of the metabolic imbalances associated with PCOS[5]
Using Gly-Pro-pNA (hydrochloride) as a substrate can assess the enzymatic activity and biochemical status of dipeptidyl peptidase IV (DPP IV)in patients with rheumatoid arthritis[6]
References:
[1]. Song JJ, Wang Q, et,al. Identification of dipeptidyl peptidase-IV inhibitory peptides from mare whey protein hydrolysates. J Dairy Sci. 2017 Sep;100(9):6885-6894. doi: 10.3168/jds.2016-11828. Epub 2017 Jul 12. PMID: 28711271.
[2]. You H, Wu T, et,al. Preparation and identification of dipeptidyl peptidase IV inhibitory peptides from quinoa protein. Food Res Int. 2022 Jun;156:111176. doi: 10.1016/j.foodres.2022.111176. Epub 2022 Mar 19. PMID: 35651037.
[3]. Yeganeh F, Mousavi SMJ, et,al. Association of CD26/dipeptidyl peptidase IV mRNA level in peripheral blood mononuclear cells with disease activity and bone erosion in rheumatoid arthritis. Clin Rheumatol. 2018 Dec;37(12):3183-3190. doi: 10.1007/s10067-018-4268-y. Epub 2018 Aug 22. PMID: 30136129.
[4]. Sun AL, Deng JT, et,al. Dipeptidyl peptidase-IV is a potential molecular biomarker in diabetic kidney disease. Diab Vasc Dis Res. 2012 Oct;9(4):301-8. doi: 10.1177/1479164111434318. Epub 2012 Mar 2. PMID: 22388283.
[5]. Blauschmidt S, Greither T, et,al. Dipeptidyl peptidase 4 serum activity and concentration are increased in women with polycystic ovary syndrome. Clin Endocrinol (Oxf). 2017 Dec;87(6):741-747. doi: 10.1111/cen.13444. Epub 2017 Sep 13. PMID: 28799235.
[6]. Mavropoulos JC, Cuchacovich M, et,al. Anti-tumor necrosis factor-alpha therapy augments dipeptidyl peptidase IV activity and decreases autoantibodies to GRP78/BIP and phosphoglucose isomerase in patients with rheumatoid arthritis. J Rheumatol. 2005 Nov;32(11):2116-24. PMID: 16265688.
| Cell experiment [1]: | |
Cell lines | Mare whey protein hydrolysates |
Preparation Method | 20 uL of sample solution and 100 uL of substrate solution (H- Gly-Pro-pNA (hydrochloride), final concentration 0.5 mM) were added. Enzyme reaction was started by adding DPP-IV. The absorbance at 405 nm was monitored after the microplate was incubated at 37°C for 0.5 h. |
Reaction Conditions | Final concentration 0.5 mM ,37 °C for 0.5 h. |
Applications | The degradation rate of the substrate Gly-Pro-pNA (hydrochloride) of DPP-IV was slowed by the addition of Mare Whey proteins. |
References: | |
| Cas No. | 103213-34-9 | SDF | |
| المرادفات | Gly-Pro pnitroanilide, GP-pNA | ||
| Chemical Name | glycyl-N-(4-nitrophenyl)-L-prolinamide, monohydrochloride | ||
| Canonical SMILES | O=C(CN)N1CCC[C@H]1C(NC2=CC=C([N+]([O-])=O)C=C2)=O.Cl | ||
| Formula | C13H16N4O4.HCl | M.Wt | 328.8 |
| الذوبان | 30mg/mL in DMSO, 30mg/mL in DMF, 30mg/mL in Ethanol | Storage | Store at -20°C,stored under nitrogen |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.0414 mL | 15.2068 mL | 30.4136 mL |
| 5 mM | 0.6083 mL | 3.0414 mL | 6.0827 mL |
| 10 mM | 0.3041 mL | 1.5207 mL | 3.0414 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
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- Purity: >98.00%
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