Glycochenodeoxycholic Acid-d4 (Synonyms: GCDCA-d4) |
| رقم الكتالوجGC47408 |
Glycochenodeoxycholic Acid-d4 (Chenodeoxycholylglycine-d4) هو حمض الديوتيريوم المسمى حمض Glycochenodeoxycholic. حمض Glycochenodeoxycholic (Chenodeoxycholylglycine) هو حمض صفراوي يتكون في الكبد من chenodeoxycholate و glycine. يعمل كمنظف لإذابة الدهون من أجل الامتصاص ويتم امتصاصه في حد ذاته. يحفز حمض Glycochenodeoxycholic (Chenodeoxycholylglycine) موت الخلايا المبرمج في الكبد.
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Cas No.: 1201918-16-2
Sample solution is provided at 25 µL, 10mM.
An internal standard for the quantification of glycochenodeoxycholic acid by GC- or LC-MS. GCDCA is a glycine-conjugated form of the primary bile acid chenodeoxycholic acid .1 It reduces formation of cholic acid in primary human hepatocytes when used at a concentration of 100 µM.2 GCDCA (50, 75, and 100 µM) reduces the number LC3 puncta, a marker of autophagy, and is cytotoxic to L-02 hepatocytes.1 GCDCA (50 µM) induces apoptosis in isolated rat hepatocytes, an effect that can be blocked by the protein kinase C (PKC) inhibitor chelerythrine .3 Fecal levels of GCDCA are decreased in a rat model of high-fat diet-induced obesity compared with rats fed a normal diet.4
1.Lan, W., Chen, Z., Chen, Y., et al.Glycochenodeoxycholic acid impairs transcription factor E3-dependent autophagy-lysosome machinery by disrupting reactive oxygen species homeostasis in L02 cellsToxicol. Lett.33111-21(2020) 2.Ellis, E., Axelson, M., Abrahamsson, A., et al.Feedback regulation of bile acid synthesis in primary human hepatocytes: Evidence that CDCA is the strongest inhibitorHepatology38(4)930-938(2003) 3.Gonzalez, B., Fisher, C., and Rosser, B.G.Glycochenodeoxycholic acid (GCDC) induced hepatocyte apoptosis is associated with early modulation of intracellular PKC activityMol. Cell. Biochem.207(1-2)19-27(2000) 4.Lin, H., An, Y., Tang, H., et al.Alterations of bile acids and gut microbiota in obesity induced by high fat diet in rat modelJ. Agric. Food Chem.67(13)3624-3632(2019)
| Cas No. | 1201918-16-2 | SDF | |
| المرادفات | GCDCA-d4 | ||
| Canonical SMILES | C[C@H](CCC(NCC(O)=O)=O)[C@@]1([H])CC[C@@]2([H])[C@]3([H])[C@H](O)C[C@]4([H])C([2H])([2H])[C@H](O)C([2H])([2H])C[C@]4(C)[C@@]3([H])CC[C@@]21C | ||
| Formula | C26H39D4NO5 | M.Wt | 453.7 |
| الذوبان | DMF: 10 mg/ml,DMSO: 10 mg/ml,DMSO:PBS(pH 7.2) (1:4): 0.2 mg/ml,Ethanol: 1 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.2041 mL | 11.0205 mL | 22.041 mL |
| 5 mM | 0.4408 mL | 2.2041 mL | 4.4082 mL |
| 10 mM | 0.2204 mL | 1.102 mL | 2.2041 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 26 reference(s) in Google Scholar.)
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